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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 5, Iss. 3 — Feb. 10, 2010

Effects of primary aberrations on the fluorescence depletion patterns of STED microscopy

Suhui Deng, Li Liu, Ya Cheng, Ruxin Li, and Zhizhan Xu  »View Author Affiliations


Optics Express, Vol. 18, Issue 2, pp. 1657-1666 (2010)
http://dx.doi.org/10.1364/OE.18.001657


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Abstract

Effects of primary aberrations including spherical aberration, coma and astigmatism on the three fluorescence depletion patterns mainly used in stimulated emission of depletion (STED) microscopy are investigated by using vectorial integral. The three depletion patterns are created by inserting a vortex phase plate, a central half-wavelength plate or a semi-circular half-wavelength mask within Gaussian beam respectively. Attention is given to the modification of the shape, peak intensity, the central intensity of the dark hole and the hole size of these depletion patterns in the presence of primary aberrations.

© 2010 OSA

OCIS Codes
(050.1960) Diffraction and gratings : Diffraction theory
(180.0180) Microscopy : Microscopy
(350.5730) Other areas of optics : Resolution

ToC Category:
Microscopy

History
Original Manuscript: November 12, 2009
Revised Manuscript: December 29, 2009
Manuscript Accepted: December 30, 2009
Published: January 14, 2010

Virtual Issues
Vol. 5, Iss. 3 Virtual Journal for Biomedical Optics

Citation
Suhui Deng, Li Liu, Ya Cheng, Ruxin Li, and Zhizhan Xu, "Effects of primary aberrations on the fluorescence depletion patterns of STED microscopy," Opt. Express 18, 1657-1666 (2010)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=oe-18-2-1657


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