Multiplane imaging and three dimensional nanoscale particle tracking in biological microscopy

doi:10.1364/OE.18.000877

Multiplane imaging and three dimensional nanoscale particle tracking in biological microscopy

Paul A. Dalgarno, Heather I. C. Dalgarno, Aurélie Putoud, Robert Lambert, Lynn Paterson, David C. Logan, David P. Towers, Richard J. Warburton, and Alan H. Greenaway »View Author Affiliations

Optics Express, Vol. 18, Issue 2, pp. 877-884 (2010)
http://dx.doi.org/10.1364/OE.18.000877

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Abstract

A conventional microscope produces a sharp image from just a single object-plane. This is often a limitation, notably in cell biology. We present a microscope attachment which records sharp images from several object-planes simultaneously. The key concept is to introduce a distorted diffraction grating into the optical system, establishing an order-dependent focussing power in order to generate several images, each arising from a different object-plane. We exploit this multiplane imaging not just for bio-imaging but also for nano-particle tracking, achieving ~10 nm z position resolution by parameterising the images with an image sharpness metric.

OCIS Codes
(050.0050) Diffraction and gratings : Diffraction and gratings
(050.1950) Diffraction and gratings : Diffraction gratings
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(170.6900) Medical optics and biotechnology : Three-dimensional microscopy
(180.0180) Microscopy : Microscopy
(180.6900) Microscopy : Three-dimensional microscopy

ToC Category:
Microscopy

History
Original Manuscript: October 23, 2009
Revised Manuscript: December 17, 2009
Manuscript Accepted: December 21, 2009
Published: January 6, 2010

Virtual Issues
Vol. 5, Iss. 3 Virtual Journal for Biomedical Optics

Citation
Paul A. Dalgarno, Heather I. C. Dalgarno, Aurélie Putoud, Robert Lambert, Lynn Paterson, David C. Logan, David P. Towers, Richard J. Warburton, and Alan H. Greenaway, "Multiplane imaging and three dimensional nanoscale particle tracking in biological microscopy," Opt. Express 18, 877-884 (2010)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=oe-18-2-877

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References

D. J. Stephens and V. J. Allan, “Light microscopy techniques for live cell imaging,” Science 300(5616), 82–86 (2003). [CrossRef] [PubMed]
M. Eisenstein, “Something to see,” Nature 443(7114), 1017–1021 (2006). [PubMed]
G. Seisenberger, M. U. Ried, T. Endress, H. Büning, M. Hallek, and C. Bräuchle, “Real-time single-molecule imaging of the infection pathway of an adeno-associated virus,” Science 294(5548), 1929–1932 (2001). [CrossRef] [PubMed]
M. G. L. Gustafsson, “Nonlinear structured-illumination microscopy: wide-field fluorescence imaging with theoretically unlimited resolution,” Proc. Natl. Acad. Sci. U.S.A. 102(37), 13081–13086 (2005). [CrossRef] [PubMed]
M. J. Cole, J. Siegel, S. E. D. Webb, R. Jones, K. Dowling, P. M. W. French, M. J. Lever, L. O. D. Sucharov, M. A. A. Neil, R. Juskaitis, and T. Wilson, “Whole-field optically sectioned fluorescence lifetime imaging,” Opt. Lett. 25(18), 1361–1363 (2000). [CrossRef]
P. J. Verveer, J. Swoger, F. Pampaloni, K. Greger, M. Marcello, and E. H. K. Stelzer, “High-resolution three-dimensional imaging of large specimens with light sheet-based microscopy,” Nat. Methods 4(4), 311–313 (2007). [PubMed]
V. Westphal, S. O. Rizzoli, M. A. Lauterbach, D. Kamin, R. Jahn, and S. W. Hell, “Video-rate far-field optical nanoscopy dissects synaptic vesicle movement,” Science 320(5873), 246–249 (2008). [CrossRef] [PubMed]
J. Rosen and G. Brooker, “Non-scanning motionless fluorescence three-dimensional holographic microscopy,” Nat. Photonics 2(3), 190–195 (2008). [CrossRef]
W. Xu, M. H. Jericho, H. J. Kreuzer, and I. A. Meinertzhagen, “Tracking particles in four dimensions with in-line holographic microscopy,” Opt. Lett. 28(3), 164–166 (2003). [CrossRef] [PubMed]
X. Qu, D. Wu, L. Mets, and N. F. Scherer, “Nanometer-localized multiple single-molecule fluorescence microscopy,” Proc. Natl. Acad. Sci. U.S.A. 101(31), 11298–11303 (2004). [CrossRef] [PubMed]
L. Holtzer, T. Meckel, and T. Schmidt, “Nanometric three-dimensional tracking of individual quantum dots in cells,” Appl. Phys. Lett. 90(5), 053902–053904 (2007). [CrossRef]
M. Speidel, A. Jonás, and E. L. Florin, “Three-dimensional tracking of fluorescent nanoparticles with subnanometer precision by use of off-focus imaging,” Opt. Lett. 28(2), 69–71 (2003). [CrossRef] [PubMed]
G. A. Lessard, P. M. Goodwin, and J. H. Werner, “Three-dimensional tracking of individual quantum dots,” Appl. Phys. Lett. 91(22), 224106–224103 (2007). [CrossRef]
P. M. Blanchard and A. H. Greenaway, “Simultaneous multiplane imaging with a distorted diffraction grating,” Appl. Opt. 38(32), 6692–6699 (1999). [CrossRef]
M. F. Juette, T. J. Gould, M. D. Lessard, M. J. Mlodzianoski, B. S. Nagpure, B. T. Bennett, S. T. Hess, and J. Bewersdorf, “Three-dimensional sub-100 nm resolution fluorescence microscopy of thick samples,” Nat. Methods 5(6), 527–529 (2008). [CrossRef] [PubMed]
P. Prabhat, S. Ram, E. S. Ward, and R. J. Ober, “Simultaneous imaging of different focal planes in fluorescence microscopy for the study of cellular dynamics in three dimensions,” IEEE Trans. Nanobioscience 3(4), 237–242 (2004). [CrossRef]
S. Djidel, J. K. Gansel, H. I. Campbell, and A. H. Greenaway, “High-speed, 3-dimensional, telecentric imaging,” Opt. Express 14(18), 8269–8277 (2006). [CrossRef] [PubMed]
I. Scott and D. C. Logan, “Mitochondrial morphology transition is an early indicator of subsequent cell death in Arabidopsis,” New Phytol. 177(1), 90–101 (2008).
R. A. Muller and A. Buffington, “Real-Time Correction of Atmospherically Degraded Telescope Images through Image Sharpening,” J. Opt. Soc. Am. 64(9), 1200–1210 (1974). [CrossRef]
P. M. Blanchard and A. H. Greenaway, “Broadband simultaneous multiplane imaging,” Opt. Commun. 183(1-4), 29–36 (2000). [CrossRef]

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[1] D. J. Stephens and V. J. Allan, “Light microscopy techniques for live cell imaging,” Science 300(5616), 82–86 (2003). [CrossRef] [PubMed]

[2] M. Eisenstein, “Something to see,” Nature 443(7114), 1017–1021 (2006). [PubMed]

[3] G. Seisenberger, M. U. Ried, T. Endress, H. Büning, M. Hallek, and C. Bräuchle, “Real-time single-molecule imaging of the infection pathway of an adeno-associated virus,” Science 294(5548), 1929–1932 (2001). [CrossRef] [PubMed]

[4] M. G. L. Gustafsson, “Nonlinear structured-illumination microscopy: wide-field fluorescence imaging with theoretically unlimited resolution,” Proc. Natl. Acad. Sci. U.S.A. 102(37), 13081–13086 (2005). [CrossRef] [PubMed]

[5] M. J. Cole, J. Siegel, S. E. D. Webb, R. Jones, K. Dowling, P. M. W. French, M. J. Lever, L. O. D. Sucharov, M. A. A. Neil, R. Juskaitis, and T. Wilson, “Whole-field optically sectioned fluorescence lifetime imaging,” Opt. Lett. 25(18), 1361–1363 (2000). [CrossRef]

[6] P. J. Verveer, J. Swoger, F. Pampaloni, K. Greger, M. Marcello, and E. H. K. Stelzer, “High-resolution three-dimensional imaging of large specimens with light sheet-based microscopy,” Nat. Methods 4(4), 311–313 (2007). [PubMed]

[7] V. Westphal, S. O. Rizzoli, M. A. Lauterbach, D. Kamin, R. Jahn, and S. W. Hell, “Video-rate far-field optical nanoscopy dissects synaptic vesicle movement,” Science 320(5873), 246–249 (2008). [CrossRef] [PubMed]

[8] J. Rosen and G. Brooker, “Non-scanning motionless fluorescence three-dimensional holographic microscopy,” Nat. Photonics 2(3), 190–195 (2008). [CrossRef]

[9] W. Xu, M. H. Jericho, H. J. Kreuzer, and I. A. Meinertzhagen, “Tracking particles in four dimensions with in-line holographic microscopy,” Opt. Lett. 28(3), 164–166 (2003). [CrossRef] [PubMed]

[10] X. Qu, D. Wu, L. Mets, and N. F. Scherer, “Nanometer-localized multiple single-molecule fluorescence microscopy,” Proc. Natl. Acad. Sci. U.S.A. 101(31), 11298–11303 (2004). [CrossRef] [PubMed]

[11] L. Holtzer, T. Meckel, and T. Schmidt, “Nanometric three-dimensional tracking of individual quantum dots in cells,” Appl. Phys. Lett. 90(5), 053902–053904 (2007). [CrossRef]

[12] M. Speidel, A. Jonás, and E. L. Florin, “Three-dimensional tracking of fluorescent nanoparticles with subnanometer precision by use of off-focus imaging,” Opt. Lett. 28(2), 69–71 (2003). [CrossRef] [PubMed]

[13] G. A. Lessard, P. M. Goodwin, and J. H. Werner, “Three-dimensional tracking of individual quantum dots,” Appl. Phys. Lett. 91(22), 224106–224103 (2007). [CrossRef]

[14] P. M. Blanchard and A. H. Greenaway, “Simultaneous multiplane imaging with a distorted diffraction grating,” Appl. Opt. 38(32), 6692–6699 (1999). [CrossRef]

[15] M. F. Juette, T. J. Gould, M. D. Lessard, M. J. Mlodzianoski, B. S. Nagpure, B. T. Bennett, S. T. Hess, and J. Bewersdorf, “Three-dimensional sub-100 nm resolution fluorescence microscopy of thick samples,” Nat. Methods 5(6), 527–529 (2008). [CrossRef] [PubMed]

[16] P. Prabhat, S. Ram, E. S. Ward, and R. J. Ober, “Simultaneous imaging of different focal planes in fluorescence microscopy for the study of cellular dynamics in three dimensions,” IEEE Trans. Nanobioscience 3(4), 237–242 (2004). [CrossRef]

[17] S. Djidel, J. K. Gansel, H. I. Campbell, and A. H. Greenaway, “High-speed, 3-dimensional, telecentric imaging,” Opt. Express 14(18), 8269–8277 (2006). [CrossRef] [PubMed]

[18] I. Scott and D. C. Logan, “Mitochondrial morphology transition is an early indicator of subsequent cell death in Arabidopsis,” New Phytol. 177(1), 90–101 (2008).

[19] R. A. Muller and A. Buffington, “Real-Time Correction of Atmospherically Degraded Telescope Images through Image Sharpening,” J. Opt. Soc. Am. 64(9), 1200–1210 (1974). [CrossRef]

[20] P. M. Blanchard and A. H. Greenaway, “Broadband simultaneous multiplane imaging,” Opt. Commun. 183(1-4), 29–36 (2000). [CrossRef]

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Multiplane imaging and three dimensional nanoscale particle tracking in biological microscopy