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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 5, Iss. 14 — Nov. 16, 2010

Depth-resolved holographic reconstructions by three-dimensional deconvolution

Tatiana Latychevskaia, Fabian Gehri, and Hans-Werner Fink  »View Author Affiliations

Optics Express, Vol. 18, Issue 21, pp. 22527-22544 (2010)

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Methods of three-dimensional deconvolution with a point-spread function as frequently employed in optical microscopy to reconstruct true three-dimensional distribution of objects are extended to holographic reconstructions. Two such schemes have been developed and are discussed: an instant deconvolution using the Wiener filter as well as an iterative deconvolution routine. The instant 3d-deconvolution can be applied to restore the positions of volume-spread objects such as small particles. The iterative deconvolution can be applied to restore the distribution of complex and extended objects. Simulated and experimental examples are presented and demonstrate artifact and noise free three-dimensional reconstructions from a single two-dimensional holographic record.

© 2010 OSA

OCIS Codes
(100.1830) Image processing : Deconvolution
(180.6900) Microscopy : Three-dimensional microscopy
(090.1995) Holography : Digital holography

ToC Category:
Image Processing

Original Manuscript: August 30, 2010
Revised Manuscript: September 27, 2010
Manuscript Accepted: October 2, 2010
Published: October 8, 2010

Virtual Issues
Vol. 5, Iss. 14 Virtual Journal for Biomedical Optics

Tatiana Latychevskaia, Fabian Gehri, and Hans-Werner Fink, "Depth-resolved holographic reconstructions by three-dimensional deconvolution," Opt. Express 18, 22527-22544 (2010)

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