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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 6, Iss. 1 — Jan. 3, 2011

A line scanned light-sheet microscope with phase shaped self-reconstructing beams

Florian O. Fahrbach and Alexander Rohrbach  »View Author Affiliations

Optics Express, Vol. 18, Issue 23, pp. 24229-24244 (2010)

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We recently demonstrated that Microscopy with Self-Reconstructing Beams (MISERB) increases both image quality and penetration depth of illumination beams in strongly scattering media. Based on the concept of line scanned light-sheet microscopy, we present an add-on module to a standard inverted microscope using a scanned beam that is shaped in phase and amplitude by a spatial light modulator. We explain technical details of the setup as well as of the holograms for the creation, positioning and scaling of static light-sheets, Gaussian beams and Bessel beams. The comparison of images from identical sample areas illuminated by different beams allows a precise assessment of the interconnection between beam shape and image quality. The superior propagation ability of Bessel beams through inhomogeneous media is demonstrated by measurements on various scattering media.

© 2010 OSA

OCIS Codes
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(230.6120) Optical devices : Spatial light modulators
(260.2710) Physical optics : Inhomogeneous optical media

ToC Category:

Original Manuscript: September 7, 2010
Manuscript Accepted: October 13, 2010
Published: November 4, 2010

Virtual Issues
Vol. 6, Iss. 1 Virtual Journal for Biomedical Optics

Florian O. Fahrbach and Alexander Rohrbach, "A line scanned light-sheet microscope with phase shaped self-reconstructing beams," Opt. Express 18, 24229-24244 (2010)

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