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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 5, Iss. 5 — Mar. 17, 2010

Imaging gold nanoparticles in living cell environments using heterodyne digital holographic microscopy

N. Warnasooriya, F. Joud, P. Bun, G. Tessier, M. Coppey-Moisan, P. Desbiolles, M. Atlan, M. Abboud, and M. Gross  »View Author Affiliations


Optics Express, Vol. 18, Issue 4, pp. 3264-3273 (2010)
http://dx.doi.org/10.1364/OE.18.003264


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Abstract

This paper describes an imaging microscopic technique based on heterodyne digital holography where subwavelength-sized gold colloids can be imaged in cell environments. Surface cellular receptors of 3T3 mouse fibroblasts are labeled with 40 nm gold nanoparticles, and the biological specimen is imaged in a total internal reflection configuration with holographic microscopy. Due to a higher scattering efficiency of the gold nanoparticles versus that of cellular structures, accurate localization of a gold marker is obtained within a 3D mapping of the entire sample’s scattered field, with a lateral precision of 5 nm and 100 nm in the x,y and in the z directions respectively, demonstrating the ability of holographic microscopy to locate nanoparticles in living cell environments.

© 2010 Optical Society of America

OCIS Codes
(170.0180) Medical optics and biotechnology : Microscopy
(170.1530) Medical optics and biotechnology : Cell analysis
(260.6970) Physical optics : Total internal reflection
(090.1995) Holography : Digital holography

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: November 25, 2009
Revised Manuscript: January 19, 2010
Manuscript Accepted: January 21, 2010
Published: February 1, 2010

Virtual Issues
Vol. 5, Iss. 5 Virtual Journal for Biomedical Optics

Citation
N. Warnasooriya, F. Joud, P. Bun, G. Tessier, M. Coppey-Moisan, P. Desbiolles, M. Atlan, M. Abboud, and M. Gross, "Imaging gold nanoparticles in living cell environments using heterodyne digital holographic microscopy," Opt. Express 18, 3264-3273 (2010)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=oe-18-4-3264


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