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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 5, Iss. 5 — Mar. 17, 2010

Imaging live cell membranes via surface plasmon-enhanced fluorescence and phase microscopy

Ruei-Yu He, Chun-Yu Lin, Yuan-Deng Su, Kuo-Chih Chiu, Nan-Shan Chang, Hua-Lin Wu, and Shean-Jen Chen  »View Author Affiliations


Optics Express, Vol. 18, Issue 4, pp. 3649-3659 (2010)
http://dx.doi.org/10.1364/OE.18.003649


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Abstract

This paper demonstrates the first combination for wide-field surface plasmon (SP) phase microscopy and SP-enhanced fluorescence microscopy to image living cells’ contacts on the surface of a bio-substrate simultaneously. The phase microscopy with a phase-shift interferometry and common-path optical setup can provide high-sensitivity phase information in long-term stability. Simultaneously, the fluorescence microscopy with the enhancement of a local electromagnetic field can supply bright fluorescent images. The combined microscope imposes a high numerical aperture objective upon the excitation of surface plasmon through a silver film with a thickness of 30 nm. The developed SP microscope is successfully applied to the real-time bright observation of the transfected fluorescence of living cells localized near the cell membrane on the bio-substrate and the high-sensitivity phase image of the cell-substrate contacts at the same time.

© 2010 OSA

OCIS Codes
(170.1530) Medical optics and biotechnology : Cell analysis
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(240.6680) Optics at surfaces : Surface plasmons

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: January 5, 2010
Revised Manuscript: January 31, 2010
Manuscript Accepted: February 3, 2010
Published: February 4, 2010

Virtual Issues
Vol. 5, Iss. 5 Virtual Journal for Biomedical Optics

Citation
Ruei-Yu He, Chun-Yu Lin, Yuan-Deng Su, Kuo-Chih Chiu, Nan-Shan Chang, Hua-Lin Wu, and Shean-Jen Chen, "Imaging live cell membranes via surface plasmon-enhanced fluorescence and phase microscopy," Opt. Express 18, 3649-3659 (2010)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=oe-18-4-3649


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References

  1. G. A. Truskey, J. S. Burmeister, E. Grapa, and W. M. Reichert, “Total internal reflection fluorescence microscopy (TIRFM). II. Topographical mapping of relative cell/substratum separation distances,” J. Cell Sci. 103(Pt 2), 491–499 (1992). [PubMed]
  2. S. E. Sund and D. Axelrod, “Actin dynamics at the living cell submembrane imaged by total internal reflection fluorescence photobleaching,” Biophys. J. 79(3), 1655–1669 (2000). [CrossRef] [PubMed]
  3. W. J. Betz, F. Mao, and C. B. Smith, “Imaging exocytosis and endocytosis,” Curr. Opin. Neurobiol. 6(3), 365–371 (1996). [CrossRef] [PubMed]
  4. D. Axelrod, “Total internal reflection fluorescence microscopy in cell biology,” Methods Enzymol. 361, 1–33 (2003). [CrossRef] [PubMed]
  5. K. F. Giebel, C. Bechinger, S. Herminghaus, M. Riedel, P. Leiderer, U. Weiland, and M. Bastmeyer, “Imaging of cell/substrate contacts of living cells with surface plasmon resonance microscopy,” Biophys. J. 76(1), 509–516 (1999). [CrossRef] [PubMed]
  6. H. Raether, Surface Plasmons on Smooth and Rough Surfaces and on Gratings (Springer, 1998).
  7. B. Huang, F. Yu, and R. N. Zare, “Surface plasmon resonance imaging using a high numerical aperture microscope objective,” Anal. Chem. 79(7), 2979–2983 (2007). [CrossRef] [PubMed]
  8. M. M. A. Jamil, M. C. T. Denyer, M. Youseffi, S. T. Britland, S. Liu, C. W. See, M. G. Somekh, and J. Zhang, “Imaging of the cell surface interface using objective coupled widefield surface plasmon microscopy,” J. Struct. Biol. 164(1), 75–80 (2008). [CrossRef] [PubMed]
  9. K. J. Moh, X. C. Yuan, J. Bu, S. W. Zhu, and B. Z. Gao, “Surface plasmon resonance imaging of cell-substrate contacts with radially polarized beams,” Opt. Express 16(25), 20734–20741 (2008). [CrossRef] [PubMed]
  10. Y. D. Su, S. J. Chen, and T. L. Yeh, “Common-path phase-shift interferometry surface plasmon resonance imaging system,” Opt. Lett. 30(12), 1488–1490 (2005). [CrossRef] [PubMed]
  11. K. H. Lee, Y. D. Su, S. J. Chen, F. G. Tseng, and G. B. Lee, “Microfluidic systems integrated with two-dimensional surface plasmon resonance phase imaging systems for microarray immunoassay,” Biosens. Bioelectron. 23(4), 466–472 (2007). [CrossRef] [PubMed]
  12. R. Y. He, G. L. Chang, H. L. Wu, C. H. Lin, K. C. Chiu, Y. D. Su, and S. J. Chen, “Enhanced live cell membrane imaging using surface plasmon-enhanced total internal reflection fluorescence microscopy,” Opt. Express 14(20), 9307–9316 (2006). [CrossRef] [PubMed]
  13. R.-Y. He, Y.-D. Su, K.-C. Cho, C.-Y. Lin, N.-S. Chang, C.-H. Chang, and S.-J. Chen, “Surface plasmon-enhanced two-photon fluorescence microscopy for live cell membrane imaging,” Opt. Express 17(8), 5987–5997 (2009). [CrossRef] [PubMed]
  14. A. W. Peterson, M. Halter, A. Tona, K. Bhadriraju, and A. L. Plant, “Surface plasmon resonance imaging of cells and surface-associated fibronectin,” BMC Cell Biol. 10(1), 16 (2009). [CrossRef] [PubMed]
  15. B. Rothenhäusler and W. Knoll, “Interferometric determination of the complex wave vector of plasmon surface polaritons,” J. Opt. Soc. Am. B 5(7), 1401–1405 (1988). [CrossRef]
  16. W. Knoll, “Optical characterization of organic thin films and interfaces with evanescent waves,” Mat. Res. Soc. Bulletin. 16, 29–39 (1991).
  17. C. D. Geddes and J. R. Lakowicz, “Metal-enhanced fluorescence,” J. Fluoresc. 12(2), 121–129 (2002). [CrossRef]
  18. J. R. Lakowicz, “Radiative decay engineering: biophysical and biomedical applications,” Anal. Biochem. 298(1), 1–24 (2001). [CrossRef] [PubMed]
  19. N. S. Chang, N. Pratt, J. Heath, L. Schultz, D. Sleve, G. B. Carey, and N. Zevotek, “Hyaluronidase induction of a WW domain-containing oxidoreductase that enhances tumor necrosis factor cytotoxicity,” J. Biol. Chem. 276(5), 3361–3370 (2001). [CrossRef]
  20. N. S. Chang, L. J. Hsu, Y. S. Lin, F. J. Lai, and H. M. Sheu, “WW domain-containing oxidoreductase: a candidate tumor suppressor,” Trends Mol. Med. 13(1), 12–22 (2007). [CrossRef]
  21. C. E. H. Berger, R. P. H. Kooyman, and J. Greve, “Resolution in surface plasmon microscopy,” Rev. Sci. Instrum. 65(9), 2829–2836 (1994). [CrossRef]
  22. P. Anger, P. Bharadwaj, and L. Novotny, “Enhancement and quenching of single-molecule fluorescence,” Phys. Rev. Lett. 96(11), 113002 (2006). [CrossRef] [PubMed]
  23. P. Hariharan, B. F. Oreb, and T. Eiju, “Digital phase-shifting interferometry: a simple error-compensating phase calculation algorithm,” Appl. Opt. 26(13), 2504–2505 (1987). [CrossRef] [PubMed]
  24. J.-J. Chyou, S.-J. Chen, and Y.-K. Chen, “Two-dimensional phase unwrapping with a multichannel least-mean-square algorithm,” Appl. Opt. 43(30), 5655–5661 (2004). [CrossRef] [PubMed]
  25. Q. Hong, L. J. Hsu, L. Schultz, N. Pratt, J. Mattison, and N. S. Chang, “Zfra affects TNF-mediated cell death by interacting with death domain protein TRADD and negatively regulates the activation of NF-kappaB, JNK1, p53 and WOX1 during stress response,” BMC Mol. Biol. 8(1), 50 (2007). [CrossRef] [PubMed]
  26. R. M. Fulbright and D. Axelrod, “Dynamics of nonspecific adsorption of insulin to erythrocyte membranes,” J. Fluoresc. 3(1), 1–16 (1993). [CrossRef]
  27. B. Rothenhäusler and W. Knoll, “Surface-plasmon microscopy,” Nature 332(6165), 615–617 (1988). [CrossRef]
  28. M. C. Boffa, B. Burke, and C. C. Haudenschild, “Preservation of thrombomodulin antigen on vascular and extravascular surfaces,” J. Histochem. Cytochem. 35(11), 1267–1276 (1987). [CrossRef] [PubMed]
  29. H. C. Huang, G. Y. Shi, S. J. Jiang, C. S. Shi, C. M. Wu, H. Y. Yang, and H. L. Wu, “Thrombomodulin-mediated cell adhesion: involvement of its lectin-like domain,” J. Biol. Chem. 278(47), 46750–46759 (2003). [CrossRef] [PubMed]

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