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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editor: Gregory W. Faris
  • Vol. 5, Iss. 5 — Mar. 17, 2010

Time- and Spectral-resolved two-photon imaging of healthy bladder mucosa and carcinoma in situ

Riccardo Cicchi, Alfonso Crisci, Alessandro Cosci, Gabriella Nesi, Dimitrios Kapsokalyvas, Saverio Giancane, Marco Carini, and Francesco S. Pavone  »View Author Affiliations

Optics Express, Vol. 18, Issue 4, pp. 3840-3849 (2010)

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Combined non-linear imaging techniques were used to deeply image human ex-vivo fresh biopsies of bladder as well as to discriminate between healthy bladder mucosa and carcinoma in situ. Morphological examination by two-photon excited fluorescence and second-harmonic generation has shown a good agreement with corresponding common routine histology performed on the same samples. Tumor cells appeared slightly different in shape and with a smaller cellular-to-nuclear dimension ratio with respect to corresponding normal cells. Further differences between the two tissue types were found in both spectral emission and fluorescence lifetime distribution by performing temporal- and spectral- resolved analysis of fluorescence. This method may represent a promising tool to be used in a multi-photon endoscope, in a confocal endoscope or in a spectroscopic probe for in-vivo optical diagnosis of bladder cancer.

© 2010 OSA

OCIS Codes
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(180.4315) Microscopy : Nonlinear microscopy

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: November 18, 2009
Revised Manuscript: January 26, 2010
Manuscript Accepted: January 28, 2010
Published: February 11, 2010

Virtual Issues
Vol. 5, Iss. 5 Virtual Journal for Biomedical Optics

Riccardo Cicchi, Alfonso Crisci, Alessandro Cosci, Gabriella Nesi, Dimitrios Kapsokalyvas, Saverio Giancane, Marco Carini, and Francesco S. Pavone, "Time- and Spectral-resolved two-photon imaging of healthy bladder mucosa and carcinoma in situ," Opt. Express 18, 3840-3849 (2010)

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