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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 5, Iss. 6 — Apr. 8, 2010

Optofluidic microscope with 3D spatial resolution

Asger Laurburg Vig, Rodolphe Marie, Eric Jensen, and Anders Kristensen  »View Author Affiliations


Optics Express, Vol. 18, Issue 5, pp. 4158-4169 (2010)
http://dx.doi.org/10.1364/OE.18.004158


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Abstract

This paper reports on-chip based optical detection with three-dimensional spatial resolution by integration of an optofluidic microscope (OFM) in a microfluidic pinched flow fractionation (PFF) separation device. This setup also enables on-chip particle image velocimetry (PIV). The position in the plane perpendicular to the flow direction and the velocity along the flow direction of separated fluorescent labeled polystyrene microspheres with diameters of 1μm, 2.1μm, 3μm and 4μm is determined by the OFM. These results are bench marked against those obtained with a PFF device using conventional fluorescence microscope readout. The size separated microspheres are detected by OFM with an accuracy of ≤0.92μm. The position in the height of the channel and the velocity of the separated microspheres are detected with an accuracy of 1.4μm and 0.08 mm/s respectively. Throughout the measurements of the height and velocity distribution, the microspheres are observed to move towards the center of the channel in regard to its height.

© 2010 Optical Society of America

OCIS Codes
(110.0180) Imaging systems : Microscopy
(110.2970) Imaging systems : Image detection systems
(120.1880) Instrumentation, measurement, and metrology : Detection
(220.4000) Optical design and fabrication : Microstructure fabrication
(110.4235) Imaging systems : Nanolithography
(130.3990) Integrated optics : Micro-optical devices

ToC Category:
Microscopy

History
Original Manuscript: October 22, 2009
Revised Manuscript: February 8, 2010
Manuscript Accepted: February 11, 2010
Published: February 17, 2010

Virtual Issues
Vol. 5, Iss. 6 Virtual Journal for Biomedical Optics

Citation
Asger Laurburg Vig, Rodolphe Marie, Eric Jensen, and Anders Kristensen, "Optofluidic microscope with 3D spatial resolution," Opt. Express 18, 4158-4169 (2010)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=oe-18-5-4158


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