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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 5, Iss. 6 — Apr. 8, 2010

High speed optical coherence microscopy with autofocus adjustment and a miniaturized endoscopic imaging probe

Aaron D. Aguirre, Juergen Sawinski, Shu-Wei Huang, Chao Zhou, Winfried Denk, and James G. Fujimoto  »View Author Affiliations


Optics Express, Vol. 18, Issue 5, pp. 4222-4239 (2010)
http://dx.doi.org/10.1364/OE.18.004222


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Abstract

Optical coherence microscopy (OCM) is a promising technique for high resolution cellular imaging in human tissues. An OCM system for high-speed en face cellular resolution imaging was developed at 1060 nm wavelength at frame rates up to 5 Hz with resolutions of < 4 µm axial and < 2 µm transverse. The system utilized a novel polarization compensation method to combat wavelength dependent source polarization and achieve broadband electro-optic phase modulation compatible with ultrahigh axial resolution. In addition, the system incorporated an auto-focusing feature that enables precise, near real-time alignment of the confocal and coherence gates in tissue, allowing user-friendly optimization of image quality during the imaging procedure. Ex vivo cellular images of human esophagus, colon, and cervix as well as in vivo results from human skin are presented. Finally, the system design is demonstrated with a miniaturized piezoelectric fiber-scanning probe which can be adapted for laparoscopic and endoscopic imaging applications.

© 2010 OSA

OCIS Codes
(170.1790) Medical optics and biotechnology : Confocal microscopy
(170.2150) Medical optics and biotechnology : Endoscopic imaging
(170.4500) Medical optics and biotechnology : Optical coherence tomography

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: November 30, 2009
Revised Manuscript: February 5, 2010
Manuscript Accepted: February 8, 2010
Published: February 17, 2010

Virtual Issues
Vol. 5, Iss. 6 Virtual Journal for Biomedical Optics

Citation
Aaron D. Aguirre, Juergen Sawinski, Shu-Wei Huang, Chao Zhou, Winfried Denk, and James G. Fujimoto, "High speed optical coherence microscopy with autofocus adjustment and a miniaturized endoscopic imaging probe," Opt. Express 18, 4222-4239 (2010)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=oe-18-5-4222


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