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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 5, Iss. 7 — Apr. 26, 2010

A multiphoton objective design with incorporated beam splitter for enhanced fluorescence collection

Jesse D. McMullen and Warren R. Zipfel  »View Author Affiliations


Optics Express, Vol. 18, Issue 6, pp. 5390-5398 (2010)
http://dx.doi.org/10.1364/OE.18.005390


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Abstract

We present a de novo design of an objective for use in multi-photon (MPM) and second harmonic generation (SHG) microscopy. This objective was designed to have a large field of view (FOV), while maintaining a moderate numerical aperture (NA) and relative straight forward construction. A dichroic beam splitter was incorporated within the objective itself allowing for an increase in the front aperture of the objective and corresponding enhancement of the solid angle of collected emission by an order of magnitude over existing designs.

© 2010 OSA

OCIS Codes
(110.2970) Imaging systems : Image detection systems
(170.0110) Medical optics and biotechnology : Imaging systems
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(180.5810) Microscopy : Scanning microscopy
(180.6900) Microscopy : Three-dimensional microscopy
(190.4180) Nonlinear optics : Multiphoton processes
(290.1350) Scattering : Backscattering
(180.4315) Microscopy : Nonlinear microscopy

ToC Category:
Microscopy

History
Original Manuscript: December 18, 2009
Revised Manuscript: January 22, 2010
Manuscript Accepted: February 1, 2010
Published: March 1, 2010

Virtual Issues
Vol. 5, Iss. 7 Virtual Journal for Biomedical Optics

Citation
Jesse D. McMullen and Warren R. Zipfel, "A multiphoton objective design with incorporated beam splitter for enhanced fluorescence collection," Opt. Express 18, 5390-5398 (2010)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=oe-18-6-5390


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