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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 5, Iss. 7 — Apr. 26, 2010

Two-photon fluorescence correlation spectroscopy of lipid-encapsulated fluorescent nanodiamonds in living cells

Yuen Yung Hui, Bailin Zhang, Yuan-Chang Chang, Cheng-Chun Chang, Huan-Cheng Chang, Jui-Hung Hsu, Karen Chang, and Fu-Hsiung Chang  »View Author Affiliations


Optics Express, Vol. 18, Issue 6, pp. 5896-5905 (2010)
http://dx.doi.org/10.1364/OE.18.005896


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Abstract

Dynamics of fluorescent diamond nanoparticles in HeLa cells has been studied with two-photon fluorescence correlation spectroscopy (FCS). Fluorescent nanodiamond (FND) is an excellent fluorescent probe for bioimaging application, but they are often trapped in endosomes after cellular uptake. The entrapment prohibits FCS from being performed in a time frame of 60 s. Herein, we show that the encapsulation of FNDs within a lipid layer enhances the diffusion of the particles in the cytoplasm by more than one order of magnitude, and particles as small as 40 nm can be probed individually with high image contrast by two-photon excited luminescence. The development of the technique together with single particle tracking through one-photon excitation allows probing of both short-term and long-term dynamics of single FNDs in living cells.

© 2010 Optical Society of America

OCIS Codes
(180.2520) Microscopy : Fluorescence microscopy
(300.6410) Spectroscopy : Spectroscopy, multiphoton
(160.4236) Materials : Nanomaterials

ToC Category:
Microscopy

History
Original Manuscript: November 19, 2009
Revised Manuscript: January 6, 2010
Manuscript Accepted: January 7, 2010
Published: March 10, 2010

Virtual Issues
Vol. 5, Iss. 7 Virtual Journal for Biomedical Optics

Citation
Yuen Yung Hui, Bailin Zhang, Yuan-Chang Chang, Cheng-Chun Chang, Huan-Cheng Chang, Jui-Hung Hsu, Karen Chang, and Fu-Hsiung Chang, "Two-photon fluorescence correlation spectroscopy of lipid-encapsulated fluorescent nanodiamonds in living cells," Opt. Express 18, 5896-5905 (2010)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=oe-18-6-5896


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