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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editor: Gregory W. Faris
  • Vol. 5, Iss. 7 — Apr. 26, 2010

Coherent anti-Stokes Raman scattering microscopy using a single-pass picosecond supercontinuum-seeded optical parametric amplifier

Chao-Yu Chung, Yen-Yin Lin, Kuo-Yu Wu, Wan-Yu Tai, Shi-Wei Chu, Yao-Chang Lee, Yeukuang Hwu, and Yin-Yu Lee  »View Author Affiliations

Optics Express, Vol. 18, Issue 6, pp. 6116-6122 (2010)

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We have demonstrated coherent anti-Stokes Raman scattering (CARS) microscopy with a single-pass picosecond supercontinuum-seeded optical parametric amplifier (SCOPA). The SCOPA was pumped by a frequency-doubled picosecond passively mode-locked Nd:YVO4 laser, and was seeded by a supercontinuum light source. Compared with the conventional experimental setups of CARS microscopy, our exposition is substantially simpler because the pump and Stokes lasers are overlapped in the SCOPA automatically and thus steered into a microscope coherently. The feasibility of this novel light source to CARS imaging was illustrated by acquiring the fundamental and overtone CARS images of the aromatic C-H stretching mode of polystyrene beads and an image of the pharynx of a C. elegans of the aliphatic C-H stretching mode.

© 2010 OSA

OCIS Codes
(190.4970) Nonlinear optics : Parametric oscillators and amplifiers
(300.6230) Spectroscopy : Spectroscopy, coherent anti-Stokes Raman scattering
(180.4315) Microscopy : Nonlinear microscopy

ToC Category:

Original Manuscript: January 6, 2010
Revised Manuscript: February 22, 2010
Manuscript Accepted: March 2, 2010
Published: March 11, 2010

Virtual Issues
Vol. 5, Iss. 7 Virtual Journal for Biomedical Optics

Chao-Yu Chung, Yen-Yin Lin, Kuo-Yu Wu, Wan-Yu Tai, Shi-Wei Chu, Yao-Chang Lee, Yeukuang Hwu, and Yin-Yu Lee, "Coherent anti-Stokes Raman scattering microscopy using a single-pass picosecond supercontinuum-seeded optical parametric amplifier," Opt. Express 18, 6116-6122 (2010)

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