OSA's Digital Library

Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 5, Iss. 7 — Apr. 26, 2010

Quantitative phase microscopy using defocusing by means of a spatial light modulator

Luis Camacho, Vicente Micó, Zeev Zalevsky, and Javier García  »View Author Affiliations


Optics Express, Vol. 18, Issue 7, pp. 6755-6766 (2010)
http://dx.doi.org/10.1364/OE.18.006755


View Full Text Article

Enhanced HTML    Acrobat PDF (1420 KB) Open Access





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

A new method for recovery the quantitative phase information of microscopic samples is presented. It is based on a spatial light modulator (SLM) and digital image processing as key elements to extract the sample’s phase distribution. By displaying a set of lenses with different focal power, the SLM produces a set of defocused images of the input sample at the CCD plane. Such recorded images are then numerically processed to retrieve phase information. This iterative process is based on the wave propagation equation and leads on a complex amplitude image containing information of both amplitude and phase distributions of the input sample diffracted wave front. The proposed configuration is a non-interferometric architecture (conventional transmission imaging mode) where no moving elements are included. Experimental results perfectly correlate with the results obtained by conventional digital holographic microscopy (DHM).

© 2010 OSA

OCIS Codes
(100.2000) Image processing : Digital image processing
(100.3010) Image processing : Image reconstruction techniques
(100.5070) Image processing : Phase retrieval
(180.0180) Microscopy : Microscopy
(090.1995) Holography : Digital holography

ToC Category:
Image Processing

History
Original Manuscript: December 22, 2009
Revised Manuscript: February 27, 2010
Manuscript Accepted: March 1, 2010
Published: March 17, 2010

Virtual Issues
Vol. 5, Iss. 7 Virtual Journal for Biomedical Optics

Citation
Luis Camacho, Vicente Micó, Zeev Zalevsky, and Javier García, "Quantitative phase microscopy using defocusing by means of a spatial light modulator," Opt. Express 18, 6755-6766 (2010)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=oe-18-7-6755


Sort:  Author  |  Year  |  Journal  |  Reset  

References

  1. D. Gabor, “A new microscopic principle,” Nature 161(4098), 777–778 (1948). [CrossRef] [PubMed]
  2. U. Schnars and W. Jüptner, “Direct recording of holograms by a CCD target and numerical reconstruction,” Appl. Opt. 33(2), 179–181 (1994). [CrossRef] [PubMed]
  3. U. Schnars, “Direct phase determination in hologram interferometry with use of digitally recorded holograms,” J. Opt. Soc. Am. A 11(7), 2011–2015 (1994). [CrossRef]
  4. L. P. Yaroslavsky, Digital Holography and Digital Image Processing: Principles, Methods, Algorithms (Kluwer, 2003).
  5. J. Garcia-Sucerquia, W. Xu, S. K. Jericho, P. Klages, M. H. Jericho, and H. J. Kreuzer, “Digital in-line holographic microscopy,” Appl. Opt. 45(5), 836–850 (2006). [CrossRef] [PubMed]
  6. V. Micó, J. García, Z. Zalevsky, and B. Javidi, “Phase-shifting Gabor holography,” Opt. Lett. 34(10), 1492–1494 (2009). [CrossRef] [PubMed]
  7. P. Marquet, B. Rappaz, P. J. Magistretti, E. Cuche, Y. Emery, T. Colomb, and Ch. Depeursinge, “Digital holographic microscopy: a noninvasive contrast imaging technique allowing quantitative visualization of living cells with subwavelength axial accuracy,” Opt. Lett. 30(5), 468–470 (2005). [CrossRef] [PubMed]
  8. F. Charrière, A. Marian, F. Montfort, J. Kuehn, T. Colomb, E. Cuche, P. Marquet, and Ch. Depeursinge, “Cell refractive index tomography by digital holographic microscopy,” Opt. Lett. 31(2), 178–180 (2006). [CrossRef] [PubMed]
  9. G. Popescu, T. Ikeda, R. R. Dasari, and M. S. Feld, “Diffraction phase microscopy for quantifying cell structure and dynamics,” Opt. Lett. 31(6), 775–777 (2006). [CrossRef] [PubMed]
  10. B. Kemper and G. von Bally, “Digital holographic microscopy for live cell applications and technical inspection,” Appl. Opt. 47(4), A52–A61 (2008). [CrossRef] [PubMed]
  11. V. Micó, Z. Zalevsky, C. Ferreira, and J. García, “Superresolution digital holographic microscopy for three-dimensional samples,” Opt. Express 16(23), 19260–19270 (2008). [CrossRef]
  12. H. Iwai, C. Fang-Yen, G. Popescu, A. Wax, K. Badizadegan, R. R. Dasari, and M. S. Feld, “Quantitative phase imaging using actively stabilized phase-shifting low-coherence interferometry,” Opt. Lett. 29(20), 2399–2401 (2004). [CrossRef] [PubMed]
  13. S. Reichelt and H. Zappe, “Combined Twyman-Green and Mach-Zehnder interferometer for microlens testing,” Appl. Opt. 44(27), 5786–5792 (2005). [CrossRef] [PubMed]
  14. Y. K. Park, G. Popescu, K. Badizadegan, R. R. Dasari, and M. S. Feld, “Fresnel particle tracing in three dimensions using diffraction phase microscopy,” Opt. Lett. 32(7), 811–813 (2007). [CrossRef] [PubMed]
  15. T. Kreis, Handbook of holographic interferometry: optical and digital methods (Wiley-VCH, 2005).
  16. I. Yamaguchi and T. Zhang, “Phase-shifting digital holography,” Opt. Lett. 22(16), 1268–1270 (1997). [CrossRef] [PubMed]
  17. I. Yamaguchi, J. Kato, S. Ohta, and J. Mizuno, “Image formation in phase-shifting digital holography and applications to microscopy,” Appl. Opt. 40(34), 6177–6186 (2001). [CrossRef]
  18. R. W. Gerchberg and W. O. Saxton, “A practical algorithm for the determination of phase from image and diffraction plane pictures,” Optik (Stuttg.) 35, 237–246 (1978).
  19. J. R. Fienup, “Phase retrieval algorithms: a comparision,” Appl. Opt. 21(15), 2758–2769 (1982). [CrossRef] [PubMed]
  20. M. R. Teague, “Deterministic phase retrieval: a Green’s function solution,” J. Opt. Soc. Am. 73(11), 1434–1441 (1983). [CrossRef]
  21. N. Streibl, “Phase imaging by the transport equation of intensity,” Opt. Commun. 49(1), 6–10 (1984). [CrossRef]
  22. M. R. Teague, “Image formation in terms of transport equation,” J. Opt. Soc. Am. A 2(11), 2019–2026 (1985). [CrossRef]
  23. G. Z. Yang, B. Z. Dong, B. Y. Gu, J. Zhuang, and O. K. Ersoy, “Gerchberg-Saxton and Yang-Gu algorithms for phase retrieval in a nonunitary transform system: a comparison,” Appl. Opt. 33(2), 209–218 (1994). [CrossRef] [PubMed]
  24. A. Barty, K. A. Nugent, D. Paganin, and A. Roberts, “Quantitative optical phase microscopy,” Opt. Lett. 23(11), 817–819 (1998). [CrossRef]
  25. Y. Zhang, G. Pedrini, W. Osten, and H. Tiziani, “Whole optical wave field reconstruction from double or multi in-line holograms by phase retrieval algorithm,” Opt. Express 11(24), 3234–3241 (2003). [CrossRef] [PubMed]
  26. G. Pedrini, W. Osten, and Y. Zhang, “Wave-front reconstruction from a sequence of interferograms recorded at different planes,” Opt. Lett. 30(8), 833–835 (2005). [CrossRef] [PubMed]
  27. P. Almoro, G. Pedrini, and W. Osten, “Complete wavefront reconstruction using sequential intensity measurements of a volume speckle field,” Appl. Opt. 45(34), 8596–8605 (2006). [CrossRef] [PubMed]
  28. P. Almoro, G. Pedrini, and W. Osten, “Aperture synthesis in phase retrieval using a volume-speckle field,” Opt. Lett. 32(7), 733–735 (2007). [CrossRef] [PubMed]
  29. A. Anand, V. K. Chhaniwal, P. Almoro, G. Pedrini, and W. Osten, “Shape and deformation measurements of 3D objects using volume speckle field and phase retrieval,” Opt. Lett. 34(10), 1522–1524 (2009). [CrossRef] [PubMed]
  30. P. F. Almoro, G. Pedrini, A. Anand, W. Osten, and S. G. Hanson, “Angular displacement and deformation analyses using a speckle-based wavefront sensor,” Appl. Opt. 48(5), 932–940 (2009). [CrossRef] [PubMed]
  31. P. Bao, F. Zhang, G. Pedrini, and W. Osten, “Phase retrieval using multiple illumination wavelengths,” Opt. Lett. 33(4), 309–311 (2008). [CrossRef] [PubMed]
  32. G. Popescu, L. P. Deflores, J. C. Vaughan, K. Badizadegan, H. Iwai, R. R. Dasari, and M. S. Feld, “Fourier phase microscopy for investigation of biological structures and dynamics,” Opt. Lett. 29(21), 2503–2505 (2004). [CrossRef] [PubMed]
  33. S. Fürhapter, A. Jesacher, S. Bernet, and M. Ritsch-Marte, “Spiral phase contrast imaging in microscopy,” Opt. Express 13(3), 689–694 (2005). [CrossRef] [PubMed]
  34. Ch. Maurer, A. Jesacher, S. Bernet, and M. Ritsch-Marte, “Phase contrast microscopy with full numerical aperture illumination,” Opt. Express 16(24), 19821–19829 (2008). [CrossRef] [PubMed]
  35. T. J. McIntyre, Ch. Maurer, S. Bernet, and M. Ritsch-Marte, “Differential interference contrast imaging using a spatial light modulator,” Opt. Lett. 34(19), 2988–2990 (2009). [CrossRef] [PubMed]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.


« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited