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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 5, Iss. 8 — Jun. 8, 2010

A simple scanless two-photon fluorescence microscope using selective plane illumination

Jonathan Palero, Susana I.C.O. Santos, David Artigas, and Pablo Loza-Alvarez  »View Author Affiliations


Optics Express, Vol. 18, Issue 8, pp. 8491-8498 (2010)
http://dx.doi.org/10.1364/OE.18.008491


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Abstract

We demonstrate a simple scanless two-photon (2p) excited fluorescence microscope based on selective plane illumination microscopy (SPIM). Optical sectioning capability is presented and depth-resolved imaging of cameleon protein in C. elegans pharyngeal muscle is implemented.

© 2010 OSA

OCIS Codes
(170.0110) Medical optics and biotechnology : Imaging systems
(170.0180) Medical optics and biotechnology : Microscopy
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(190.4180) Nonlinear optics : Multiphoton processes
(180.4315) Microscopy : Nonlinear microscopy

ToC Category:
Microscopy

History
Original Manuscript: February 26, 2010
Revised Manuscript: March 24, 2010
Manuscript Accepted: March 31, 2010
Published: April 7, 2010

Virtual Issues
Vol. 5, Iss. 8 Virtual Journal for Biomedical Optics

Citation
Jonathan Palero, Susana I.C.O. Santos, David Artigas, and Pablo Loza-Alvarez, "A simple scanless two-photon fluorescence microscope using selective plane illumination," Opt. Express 18, 8491-8498 (2010)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=oe-18-8-8491


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