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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 6, Iss. 7 — Jul. 27, 2011

In-plane rotation classification for coherent X-ray imaging of single biomolecules

Kaiqin Chu, James Evans, Nina Rohringer, Stefan Hau-Riege, Alexander Graf, Matthias Frank, Zachary J. Smith, and Stephen Lane  »View Author Affiliations


Optics Express, Vol. 19, Issue 12, pp. 11691-11704 (2011)
http://dx.doi.org/10.1364/OE.19.011691


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Abstract

We report a new classification scheme with computation complexity well within the capacity of a PC for coherent X-ray imaging of single biomolecules. In contrast to current methods, which are based on data from large scattering angles, we propose to classify the orientations of the biomolecule using data from small angle scattering, where the signals are relatively strong. Further we integrate data to form radial and azimuthal distributions of the scattering pattern to reduce the variance caused by the shot noise. Classification based on these two distributions are shown to successfully recognize not only the patterns from molecules of the same orientation but also those that differ by an in-plane rotation.

© 2011 OSA

OCIS Codes
(260.1960) Physical optics : Diffraction theory
(290.5840) Scattering : Scattering, molecules
(290.2558) Scattering : Forward scattering
(110.3200) Imaging systems : Inverse scattering

ToC Category:
X-ray Optics

History
Original Manuscript: February 15, 2011
Revised Manuscript: May 7, 2011
Manuscript Accepted: May 25, 2011
Published: June 1, 2011

Virtual Issues
Vol. 6, Iss. 7 Virtual Journal for Biomedical Optics

Citation
Kaiqin Chu, James Evans, Nina Rohringer, Stefan Hau-Riege, Alexander Graf, Matthias Frank, Zachary J. Smith, and Stephen Lane, "In-plane rotation classification for coherent X-ray imaging of single biomolecules," Opt. Express 19, 11691-11704 (2011)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=oe-19-12-11691


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