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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 6, Iss. 7 — Jul. 27, 2011

Enhanced two-photon excited fluorescence in three-dimensionally crosslinked bovine serum albumin microstructures

Keng-Chi Cho, Chi-Hsiang Lien, Chun-Yu Lin, Chia-Yuan Chang, Lynn L. H. Huang, Paul J. Campagnola, Chen Yuan Dong, and Shean-Jen Chen  »View Author Affiliations

Optics Express, Vol. 19, Issue 12, pp. 11732-11739 (2011)

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In this study, the intensity of two-photon excited fluorescence (TPEF) of xanthene dye, Rose Bengal (RB), was significantly enhanced via bovine serum albumin (BSA) microstructures fabricated by the two-photon crosslinking (TPC) technique. The RB was utilized as the photoactivator in the TPC processing and the enhanced TPEF intensity correlates with the concentration of fabricated crosslinked BSA microstructures via the power control and pulse selection of the employed femtosecond laser. As a result, fabrication of three-dimensional BSA microstructures can be simultaneously monitored by the use of TPEF intensity. The crosslinked BSA microstructures synthesized may be used as an ordered biomaterial for fluorescence enhancement.

© 2011 OSA

OCIS Codes
(160.2540) Materials : Fluorescent and luminescent materials
(190.4180) Nonlinear optics : Multiphoton processes
(220.4000) Optical design and fabrication : Microstructure fabrication

ToC Category:

Original Manuscript: March 16, 2011
Revised Manuscript: May 4, 2011
Manuscript Accepted: May 29, 2011
Published: June 1, 2011

Virtual Issues
Vol. 6, Iss. 7 Virtual Journal for Biomedical Optics

Keng-Chi Cho, Chi-Hsiang Lien, Chun-Yu Lin, Chia-Yuan Chang, Lynn L. H. Huang, Paul J. Campagnola, Chen Yuan Dong, and Shean-Jen Chen, "Enhanced two-photon excited fluorescence in three-dimensionally crosslinked bovine serum albumin microstructures," Opt. Express 19, 11732-11739 (2011)

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