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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 6, Iss. 7 — Jul. 27, 2011

Detection of malarial byproduct hemozoin utilizing its unique scattering properties

Benjamin K. Wilson, Matthew R. Behrend, Matthew P. Horning, and Michael C. Hegg  »View Author Affiliations


Optics Express, Vol. 19, Issue 13, pp. 12190-12196 (2011)
http://dx.doi.org/10.1364/OE.19.012190


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Abstract

The scattering characteristics of the malaria byproduct hemozoin, including its scattering distribution and depolarization, are modeled using Discrete Dipole Approximation (DDA) and compared to those of healthy red blood cells. Scattering (or dark-field) spectroscopy and imaging are used to identify hemozoin in fresh rodent blood samples. A new detection method is proposed and demonstrated using dark-field in conjunction with cross-polarization imaging and spectroscopy. SNRs greater than 50:1 are achieved for hemozoin in fresh blood without the addition of stains or reagents. The potential of such a detection system is discussed.

© 2011 OSA

OCIS Codes
(170.0180) Medical optics and biotechnology : Microscopy
(290.5820) Scattering : Scattering measurements

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: May 19, 2011
Manuscript Accepted: June 1, 2011
Published: June 8, 2011

Virtual Issues
Vol. 6, Iss. 7 Virtual Journal for Biomedical Optics

Citation
Benjamin K. Wilson, Matthew R. Behrend, Matthew P. Horning, and Michael C. Hegg, "Detection of malarial byproduct hemozoin utilizing its unique scattering properties," Opt. Express 19, 12190-12196 (2011)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=oe-19-13-12190


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