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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 6, Iss. 8 — Aug. 26, 2011

Analysis of multiple scattering suppression using structured laser illumination planar imaging in scattering and fluorescing media

E. Kristensson, L. Araneo, E. Berrocal, J. Manin, M. Richter, M. Aldén, and M. Linne  »View Author Affiliations

Optics Express, Vol. 19, Issue 14, pp. 13647-13663 (2011)

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The accuracy, precision and limitations of the imaging technique named Structured Laser Illumination Planar Imaging (SLIPI) have been investigated. SLIPI, which allows multiply scattered light to be diminished, has previously demonstrated improvements in image quality and contrast for spray imaging. In the current study the method is applied to a controlled confined environment consisting of a mixture of water and monodisperse polystyrene microspheres. Elastic scattering and fluorescence are studied and the results obtained when probing different particle concentrations and diameters conclusively show the advantages of SLIPI for imaging within moderately turbid media. Although the technique presents both good repeatability and agreement with the Beer-Lambert law, discrepancies in its performance were, however, discovered. Photons undergoing scattering without changing their incident trajectory cannot be discriminated and, owing to differences in scattering phase functions, probing larger particles reduces the suppression of multiply scattered light. However, in terms of visibility such behavior is beneficial as it allows denser media to be probed. It is further demonstrated that the suppression of diffuse light performs equally well regardless of whether photons propagate along the incident direction or towards the camera. In addition, this filtering process acts independently on the spatial distribution of the multiply scattered light but is limited by the finite dynamic range and unavoidable signal noise of the camera.

© 2011 OSA

OCIS Codes
(290.2200) Scattering : Extinction
(290.4210) Scattering : Multiple scattering
(290.7050) Scattering : Turbid media
(110.0113) Imaging systems : Imaging through turbid media

ToC Category:

Original Manuscript: March 30, 2011
Revised Manuscript: May 12, 2011
Manuscript Accepted: May 12, 2011
Published: June 30, 2011

Virtual Issues
Vol. 6, Iss. 8 Virtual Journal for Biomedical Optics

E. Kristensson, L. Araneo, E. Berrocal, J. Manin, M. Richter, M. Aldén, and M. Linne, "Analysis of multiple scattering suppression using structured laser illumination planar imaging in scattering and fluorescing media," Opt. Express 19, 13647-13663 (2011)

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