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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 6, Iss. 8 — Aug. 26, 2011

Analyzing speckle contrast for HiLo microscopy optimization

J. Mazzaferri, D. Kunik, J. M. Belisle, K. Singh, S. Lefrançois, and S. Costantino  »View Author Affiliations


Optics Express, Vol. 19, Issue 15, pp. 14508-14517 (2011)
http://dx.doi.org/10.1364/OE.19.014508


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Abstract

HiLo microscopy is a recently developed technique that provides both optical sectioning and fast imaging with a simple implementation and at a very low cost. The methodology combines widefield and speckled illumination images to obtain one optically sectioned image. Hence, the characteristics of such speckle illumination ultimately determine the quality of HiLo images and the overall performance of the method. In this work, we study how speckle contrast influence local variations of fluorescence intensity and brightness profiles of thick samples. We present this article as a guide to adjust the parameters of the system for optimizing the capabilities of this novel technology.

© 2011 OSA

OCIS Codes
(110.6150) Imaging systems : Speckle imaging
(170.1790) Medical optics and biotechnology : Confocal microscopy
(170.6900) Medical optics and biotechnology : Three-dimensional microscopy
(180.2520) Microscopy : Fluorescence microscopy

ToC Category:
Microscopy

History
Original Manuscript: June 1, 2011
Revised Manuscript: June 30, 2011
Manuscript Accepted: July 5, 2011
Published: July 13, 2011

Virtual Issues
Vol. 6, Iss. 8 Virtual Journal for Biomedical Optics

Citation
J. Mazzaferri, D. Kunik, J. M. Belisle, K. Singh, S. Lefrançois, and S. Costantino, "Analyzing speckle contrast for HiLo microscopy optimization," Opt. Express 19, 14508-14517 (2011)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=oe-19-15-14508


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References

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