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The effect of immersion oil in optical tweezers |
Optics Express, Vol. 19, Issue 16, pp. 14794-14800 (2011)
http://dx.doi.org/10.1364/OE.19.014794
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Abstract
Optimized optical tweezers are of great importance for biological micromanipulation. In this paper, we present a detailed electromagnetic-based calculation of the spatial intensity distribution for a laser beam focused through a high numerical aperture objective when there are several discontinuities in the optical pathway of the system. For a common case of 3 interfaces we have shown that 0.01 increase in the refractive index of the immersion medium would shift the optimal trapping depth by 3–4μm (0.2–0.6μm) for aqueous (air) medium. For the first time, We have shown that the alteration of the refractive index of the immersion medium can be also used in aerosol trapping provided that larger increase in the refractive index is considered.
© 2011 OSA
OCIS Codes
(140.7010) Lasers and laser optics : Laser trapping
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(220.1000) Optical design and fabrication : Aberration compensation
(350.4855) Other areas of optics : Optical tweezers or optical manipulation
ToC Category:
Optical Trapping and Manipulation
History
Original Manuscript: April 4, 2011
Revised Manuscript: May 15, 2011
Manuscript Accepted: May 15, 2011
Published: July 18, 2011
Virtual Issues
Vol. 6, Iss. 9 Virtual Journal for Biomedical Optics
Citation
Ali Mahmoudi and S. Nader S. Reihani, "The effect of immersion oil in optical tweezers," Opt. Express 19, 14794-14800 (2011)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=oe-19-16-14794
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