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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 6, Iss. 9 — Oct. 3, 2011

Lensfree super-resolution holographic microscopy using wetting films on a chip

Onur Mudanyali, Waheb Bishara, and Aydogan Ozcan  »View Author Affiliations


Optics Express, Vol. 19, Issue 18, pp. 17378-17389 (2011)
http://dx.doi.org/10.1364/OE.19.017378


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Abstract

We investigate the use of wetting films to significantly improve the imaging performance of lensfree pixel super-resolution on-chip microscopy, achieving < 1 µm spatial resolution over a large imaging area of ~24 mm2. Formation of an ultra-thin wetting film over the specimen effectively creates a micro-lens effect over each object, which significantly improves the signal-to-noise-ratio and therefore the resolution of our lensfree images. We validate the performance of this approach through lensfree on-chip imaging of various objects having fine morphological features (with dimensions of e.g., ≤0.5 µm) such as Escherichia coli (E. coli), human sperm, Giardia lamblia trophozoites, polystyrene micro beads as well as red blood cells. These results are especially important for the development of highly sensitive field-portable microscopic analysis tools for resource limited settings.

© 2011 OSA

OCIS Codes
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(090.1995) Holography : Digital holography

ToC Category:
Holography

History
Original Manuscript: July 5, 2011
Revised Manuscript: July 30, 2011
Manuscript Accepted: August 3, 2011
Published: August 18, 2011

Virtual Issues
Vol. 6, Iss. 9 Virtual Journal for Biomedical Optics

Citation
Onur Mudanyali, Waheb Bishara, and Aydogan Ozcan, "Lensfree super-resolution holographic microscopy using wetting films on a chip," Opt. Express 19, 17378-17389 (2011)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=oe-19-18-17378


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