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High resolution cellular imaging with nonlinear optical infrared microscopy |
Optics Express, Vol. 19, Issue 2, pp. 1378-1384 (2011)
http://dx.doi.org/10.1364/OE.19.001378
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Abstract
We developed a nonlinear optical infrared microscope exploiting a thermally induced refractive index change in the mid-infrared regime and imaged a single biological cell with high spatial resolution that was not possible in conventional infrared microscopes. A refractive index change of a sample induced by infrared (~3.5 μm) absorption was probed by a visible (633 nm) laser beam. Thus the chemical specificity stems from the spectral absorbance of specimen and the spatial resolution from the short wavelength visible radiation. A reflecting objective (NA0.5) was used to focus the infrared and visible beams on the sample plane, and the sample was raster-scanned for 2-D imaging. The high resolution beyond the infrared diffraction limit was demonstrated by imaging fine grating lines made up of epoxy grooves (830 lines/mm). The probe wavelength dependence of the spatial resolution was investigated by imaging polystyrene beads. We found that the resolution was as small as 0.7 μm with 633 nm probe wavelength.
© 2011 OSA
OCIS Codes
(110.0180) Imaging systems : Microscopy
(110.3080) Imaging systems : Infrared imaging
(190.3270) Nonlinear optics : Kerr effect
ToC Category:
Microscopy
History
Original Manuscript: November 3, 2010
Revised Manuscript: December 16, 2010
Manuscript Accepted: January 1, 2011
Published: January 12, 2011
Virtual Issues
Vol. 6, Iss. 2 Virtual Journal for Biomedical Optics
Citation
Eun Seong Lee and Jae Yong Lee, "High resolution cellular imaging with nonlinear optical infrared microscopy," Opt. Express 19, 1378-1384 (2011)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=oe-19-2-1378
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