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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 7, Iss. 1 — Jan. 4, 2012

Identification of bovine sperm head for morphometry analysis in quantitative phase-contrast holographic microscopy

P. Memmolo, G. Di Caprio, C. Distante, M. Paturzo, R. Puglisi, D. Balduzzi, A. Galli, G. Coppola, and P. Ferraro  »View Author Affiliations

Optics Express, Vol. 19, Issue 23, pp. 23215-23226 (2011)

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An investigation is reported of the identification and measurement of region of interest (ROI) in quantitative phase-contrast maps of biological cells by digital holographic microscopy. In particular, two different methods have been developed for in vitro bull sperm head morphometry analysis. We show that semen analysis can be accomplished by means of the proposed techniques . Extraction and measurement of various parameters are performed. It is demonstrated that both proposed methods are efficient to skim the data set in a preselective analysis for discarding anomalous data.

© 2011 OSA

OCIS Codes
(100.2000) Image processing : Digital image processing
(100.5070) Image processing : Phase retrieval
(170.1530) Medical optics and biotechnology : Cell analysis
(180.3170) Microscopy : Interference microscopy
(090.1995) Holography : Digital holography

ToC Category:
Image Processing

Original Manuscript: July 5, 2011
Revised Manuscript: August 11, 2011
Manuscript Accepted: August 12, 2011
Published: November 1, 2011

Virtual Issues
Vol. 7, Iss. 1 Virtual Journal for Biomedical Optics

P. Memmolo, G. Di Caprio, C. Distante, M. Paturzo, R. Puglisi, D. Balduzzi, A. Galli, G. Coppola, and P. Ferraro, "Identification of bovine sperm head for morphometry analysis in quantitative phase-contrast holographic microscopy," Opt. Express 19, 23215-23226 (2011)

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