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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 7, Iss. 1 — Jan. 4, 2012

Individual bioaerosol particle discrimination by multi-photon excited fluorescence

Denis Kiselev, Luigi Bonacina, and Jean-Pierre Wolf  »View Author Affiliations


Optics Express, Vol. 19, Issue 24, pp. 24516-24521 (2011)
http://dx.doi.org/10.1364/OE.19.024516


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Abstract

Femtosecond laser induced multi-photon excited fluorescence (MPEF) from individual airborne particles is tested for the first time for discriminating bioaerosols. The fluorescence spectra, analysed in 32 channels, exhibit a composite character originating from simultaneous two-photon and three-photon excitation at 790 nm. Simulants of bacteria aggregates (clusters of dyed polystyrene microspheres) and different pollen particles (Ragweed, Pecan, Mulberry) are clearly discriminated by their MPEF spectra. This demonstration experiment opens the way to more sophisticated spectroscopic schemes like pump-probe and coherent control.

© 2011 OSA

OCIS Codes
(190.4180) Nonlinear optics : Multiphoton processes
(280.1120) Remote sensing and sensors : Air pollution monitoring

ToC Category:
Remote Sensing

History
Original Manuscript: September 13, 2011
Revised Manuscript: October 11, 2011
Manuscript Accepted: October 11, 2011
Published: November 15, 2011

Virtual Issues
Vol. 7, Iss. 1 Virtual Journal for Biomedical Optics

Citation
Denis Kiselev, Luigi Bonacina, and Jean-Pierre Wolf, "Individual bioaerosol particle discrimination by multi-photon excited fluorescence," Opt. Express 19, 24516-24521 (2011)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=oe-19-24-24516


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