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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 7, Iss. 2 — Feb. 1, 2012

Lensless digital holography with diffuse illumination through a pseudo-random phase mask

Stefan Bernet, Walter Harm, Alexander Jesacher, and Monika Ritsch-Marte  »View Author Affiliations

Optics Express, Vol. 19, Issue 25, pp. 25113-25124 (2011)

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Microscopic imaging with a setup consisting of a pseudo-random phase mask, and an open CMOS camera, but without an imaging objective, is demonstrated. The pseudo random phase mask acts as a diffuser for an incoming laser beam, scattering a speckle pattern to a CMOS chip, which is recorded once, as a reference. A sample which is afterwards inserted somewhere in the optical beam path changes the speckle pattern. A single (non-iterative) image processing step, comparing the modified speckle pattern with the previously recorded one, generates a sharp image of the sample. After a first calibration the method works in real-time and allows quantitative imaging of complex (amplitude and phase) samples in an extended three-dimensional volume. Since no lenses are used, the method is free from lens aberrations. Compared to standard inline holography the diffuse sample illumination improves the axial sectioning capability by increasing the effective numerical aperture in the illumination path, and it suppresses the undesired twin images. For demonstration, a high resolution spatial light modulator (SLM) is programmed to act as the pseudo-random phase mask. We show experimental results, imaging microscopic biological samples, such as insects, within an extended volume at a distance of 15 cm with a transverse and longitudinal resolution of about 60 μm and 400 μm, respectively.

© 2011 OSA

OCIS Codes
(110.6150) Imaging systems : Speckle imaging
(090.1995) Holography : Digital holography

ToC Category:

Original Manuscript: September 27, 2011
Revised Manuscript: November 3, 2011
Manuscript Accepted: November 6, 2011
Published: November 23, 2011

Virtual Issues
Vol. 7, Iss. 2 Virtual Journal for Biomedical Optics

Stefan Bernet, Walter Harm, Alexander Jesacher, and Monika Ritsch-Marte, "Lensless digital holography with diffuse illumination through a pseudo-random phase mask," Opt. Express 19, 25113-25124 (2011)

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  1. D. Gabor, “A new microscopic principle,” Nature 161, 777–778 (1948). [CrossRef] [PubMed]
  2. D. Gabor, “Microscopy by reconstructed wave-fronts,” Proc. R. Soc. London Ser. A 197, 454–487 (1949). [CrossRef]
  3. I. Moon, M. Daneshpanah, A. Anand, and B. Javidi, “Cell identification with computational 3-D holographic microscopy,” Opt. Photon. News 22, 18–23 (2011). [CrossRef]
  4. T. Nomura and M. Imbe, “Single-exposure phase-shifting digital holography using a random-phase reference wave,” Opt. Lett. 35, 2281–2283 (2010). [CrossRef] [PubMed]
  5. C. Maurer, A. Schwaighofer, A. Jesacher, S. Bernet, and M. Ritsch-Marte, “Suppression of undesired diffraction orders of binary phase holograms,” Appl. Opt. 47, 3994–3998 (2008). [CrossRef] [PubMed]
  6. F. Zhang, G. Pedrini, and W. Osten, “Phase retrieval of arbitrary complex-valued fields through aperture-plane modulation,” Phys. Rev. A 75, 043805 (2007). [CrossRef]
  7. C. Kohler, F. Zhang, and W. Osten, “Characterization of a spatial light modulator and its application in phase retrieval,” Appl. Opt. 48, 4003–4008 (2009). [CrossRef] [PubMed]
  8. An explanation of iterative Fourier transform algorithms can be found for example in: B. C. Kress and P. Meyrueis (Eds.) “Digital Diffractive Optics,” 1st ed. (John Wiley & Sons, 2000) ISBN-13: 978-0-471-98447-4.
  9. A. Jesacher, S. Fürhapter, S. Bernet, and M. Ritsch-Marte, “Diffractive optical tweezers in the Fresnel regime,” Opt. Express 12, 2243–2250 (2004). [CrossRef] [PubMed]
  10. J. R. Fienup, “Reconstruction of an object from the modulus of its Fourier transform,” Opt. Lett. 3, 27–29 (1978). [CrossRef] [PubMed]
  11. J. R. Fienup, “Phase retrieval algorithms: a comparison,” Appl. Opt. 21, 2758–2769 (1982). [CrossRef] [PubMed]
  12. J. N. Cederquist, J. R. Fienup, J. C. Marron, and R. G. Paxman, “Phase retrival from experimental far-field speckle data,” Opt. Lett. 13, 619–621 (1988). [CrossRef] [PubMed]

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