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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 7, Iss. 2 — Feb. 1, 2012

Review of fluorescent standards for calibration of in situ fluorometers: Recommendations applied in coastal and ocean observing programs

Alan Earp, Christine E. Hanson, Peter J. Ralph, Vittorio E. Brando, Simon Allen, Mark Baird, Lesley Clementson, Paul Daniel, Arnold G. Dekker, Peter R.C.S. Fearns, John Parslow, Peter G. Strutton, Peter A. Thompson, Mark Underwood, Scarla Weeks, and Martina A. Doblin  »View Author Affiliations


Optics Express, Vol. 19, Issue 27, pp. 26768-26782 (2011)
http://dx.doi.org/10.1364/OE.19.026768


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Abstract

Fluorometers are widely used in ecosystem observing to monitor fluorescence signals from organic compounds, as well as to infer geophysical parameters such as chlorophyll or CDOM concentration, but measurements are susceptible to variation caused by biofouling, instrument design, sensor drift, operating environment, and calibration rigor. To collect high quality data, such sensors need frequent checking and regular calibration. In this study, a wide variety of both liquid and solid fluorescent materials were trialed to assess their suitability as reference standards for performance assessment of in situ fluorometers. Criteria used to evaluate the standards included the spectral excitation/emission responses of the materials relative to fluorescence sensors and to targeted ocean properties, the linearity of the fluorometer’s optical response with increasing concentration, stability and consistency, availability and ease of use, as well as cost. Findings are summarized as a series of recommended reference standards for sensors deployed on stationary and mobile platforms, to suit a variety of in situ coastal to ocean sensor configurations. Repeated determinations of chlorophyll scale factor using the recommended liquid standard, Fluorescein, achieved an accuracy of 2.5%. Repeated measurements with the recommended solid standard, Plexiglas Satinice® plum 4H01 DC (polymethylmethacrylate), over an 18 day period varied from the mean value by 1.0% for chlorophyll sensors and 3.3% for CDOM sensors.

© 2011 OSA

OCIS Codes
(010.0010) Atmospheric and oceanic optics : Atmospheric and oceanic optics
(010.4450) Atmospheric and oceanic optics : Oceanic optics
(120.0280) Instrumentation, measurement, and metrology : Remote sensing and sensors
(260.2510) Physical optics : Fluorescence
(150.1488) Machine vision : Calibration

ToC Category:
Atmospheric and Oceanic Optics

History
Original Manuscript: August 3, 2011
Revised Manuscript: October 6, 2011
Manuscript Accepted: October 8, 2011
Published: December 14, 2011

Virtual Issues
Vol. 7, Iss. 2 Virtual Journal for Biomedical Optics

Citation
Alan Earp, Christine E. Hanson, Peter J. Ralph, Vittorio E. Brando, Simon Allen, Mark Baird, Lesley Clementson, Paul Daniel, Arnold G. Dekker, Peter R.C.S. Fearns, John Parslow, Peter G. Strutton, Peter A. Thompson, Mark Underwood, Scarla Weeks, and Martina A. Doblin, "Review of fluorescent standards for calibration of in situ fluorometers: Recommendations applied in coastal and ocean observing programs," Opt. Express 19, 26768-26782 (2011)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=oe-19-27-26768


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