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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 6, Iss. 3 — Mar. 18, 2011

High-speed confocal fluorescence lifetime imaging microscopy (FLIM) with the analog mean delay (AMD) method

Youngjae Won, Sucbei Moon, Wenzhong Yang, Donguk Kim, Won-Taek Han, and Dug Young Kim  »View Author Affiliations


Optics Express, Vol. 19, Issue 4, pp. 3396-3405 (2011)
http://dx.doi.org/10.1364/OE.19.003396


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Abstract

We demonstrate a high-speed confocal fluorescence lifetime imaging microscopy (FLIM) whose accuracy and photon economy are as good as that of a time-correlated single photon counting (TCSPC). It is based on a new lifetime determination scheme, the analog mean delay (AMD) method. Due to the technical advantages of multiple fluorescence photon detection capability, accurate lifetime determination scheme and high photon detection efficiency, the AMD method can be the most effective method for high-speed confocal FLIM. The feasibility of real-time confocal FLIM with the AMD method has been demonstrated by observing the dynamic reaction of calcium channels in a RBL-2H3 cell with respect to 4αPDD stimulus. We have achieved the photon detection rate of 125 times faster than a conventional TCSPC based system in this experiment.

© 2011 OSA

OCIS Codes
(000.2170) General : Equipment and techniques
(170.0110) Medical optics and biotechnology : Imaging systems
(170.1790) Medical optics and biotechnology : Confocal microscopy
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(170.3650) Medical optics and biotechnology : Lifetime-based sensing
(170.6920) Medical optics and biotechnology : Time-resolved imaging

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: November 24, 2010
Revised Manuscript: January 28, 2011
Manuscript Accepted: January 28, 2011
Published: February 7, 2011

Virtual Issues
Vol. 6, Iss. 3 Virtual Journal for Biomedical Optics

Citation
Youngjae Won, Sucbei Moon, Wenzhong Yang, Donguk Kim, Won-Taek Han, and Dug Young Kim, "High-speed confocal fluorescence lifetime imaging microscopy (FLIM) with the analog mean delay (AMD) method," Opt. Express 19, 3396-3405 (2011)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=oe-19-4-3396


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