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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 6, Iss. 4 — May. 4, 2011

Numerical evaluation of temporal focusing characteristics in transparent and scattering media

Hod Dana and Shy Shoham  »View Author Affiliations

Optics Express, Vol. 19, Issue 6, pp. 4937-4948 (2011)

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Temporal focusing is a simple approach for achieving tight, optically sectioned excitation in nonlinear microscopy and multiphoton photo-manipulation. Key applications and advantages of temporal focusing involve propagation through scattering media, but the progressive broadening of the temporal focus has not been characterized. By combining a detailed geometrical optics model with Monte-Carlo scattering simulations we introduce and validate a simulation strategy for predicting temporal focusing characteristics in scattering and non-scattering media. The broadening of the temporal focus width with increasing depth in brain tissue is studied using both simulations and experiments for several key optical geometries, and an analytical approximation is found for the dependence of this broadening on the microscope’s parameters in a transparent medium. Our results indicate that a multiphoton temporal focus has radically different broadening characteristics in deep tissue than those of a spatial focus.

© 2011 OSA

OCIS Codes
(000.4430) General : Numerical approximation and analysis
(170.3660) Medical optics and biotechnology : Light propagation in tissues
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(180.6900) Microscopy : Three-dimensional microscopy
(190.4180) Nonlinear optics : Multiphoton processes

ToC Category:
Imaging Systems

Original Manuscript: December 20, 2010
Revised Manuscript: February 2, 2011
Manuscript Accepted: February 9, 2011
Published: March 1, 2011

Virtual Issues
Vol. 6, Iss. 4 Virtual Journal for Biomedical Optics

Hod Dana and Shy Shoham, "Numerical evaluation of temporal focusing characteristics in transparent and scattering media," Opt. Express 19, 4937-4948 (2011)

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