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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 6, Iss. 4 — May. 4, 2011

Label-free imaging of intracellular motility by low-coherent quantitative phase microscopy

Toyohiko Yamauchi, Hidenao Iwai, and Yutaka Yamashita  »View Author Affiliations


Optics Express, Vol. 19, Issue 6, pp. 5536-5550 (2011)
http://dx.doi.org/10.1364/OE.19.005536


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Abstract

The subject study demonstrates the imaging of cell activity by quantitatively assessing the motion of intracellular organelles and cell plasma membranes without any contrast agent. The low-coherent interferometric technique and phase-referenced phase shifting technique were integrated to reveal the depth-resolved distribution of intracellular motility. The transversal and vertical spatial resolutions were 0.56 μm and 0.93 μm, respectively, and the mechanical stability of the system was 1.2 nm. The motility of the cell was assessed by mean squared displacement (MSD) and we have compensated for the MSD by applying statistical noise analysis. Thus we show the significant change of intracellular motility after paraformaldehyde treatment in non-labeled cells.

© 2011 OSA

OCIS Codes
(170.1530) Medical optics and biotechnology : Cell analysis
(180.3170) Microscopy : Interference microscopy
(240.6648) Optics at surfaces : Surface dynamics

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: January 31, 2011
Revised Manuscript: February 28, 2011
Manuscript Accepted: February 28, 2011
Published: March 9, 2011

Virtual Issues
Vol. 6, Iss. 4 Virtual Journal for Biomedical Optics

Citation
Toyohiko Yamauchi, Hidenao Iwai, and Yutaka Yamashita, "Label-free imaging of intracellular motility by low-coherent quantitative phase microscopy," Opt. Express 19, 5536-5550 (2011)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=oe-19-6-5536


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References

  1. A. W. Partin, J. S. Schoeniger, J. L. Mohler, and D. S. Coffey, “Fourier analysis of cell motility: correlation of motility with metastatic potential,” Proc. Natl. Acad. Sci. U.S.A. 86(4), 1254–1258 (1989). [CrossRef] [PubMed]
  2. P. C. Zhang, A. M. Keleshian, and F. Sachs, “Voltage-induced membrane movement,” Nature 413(6854), 428–432 (2001). [CrossRef] [PubMed]
  3. S. Suresh, “Biomechanics and biophysics of cancer cells,” Acta Biomater. 3(4), 413–438 (2007). [CrossRef] [PubMed]
  4. T. Ikeda, G. Popescu, R. R. Dasari, and M. S. Feld, “Hilbert phase microscopy for investigating fast dynamics in transparent systems,” Opt. Lett. 30(10), 1165–1167 (2005). [CrossRef] [PubMed]
  5. G. Popescu, T. Ikeda, C. A. Best, K. Badizadegan, R. R. Dasari, and M. S. Feld, “Erythrocyte structure and dynamics quantified by Hilbert phase microscopy,” J. Biomed. Opt. 10(6), 060503 (2005). [CrossRef]
  6. G. Popescu, T. Ikeda, K. Goda, C. A. Best-Popescu, M. Laposata, S. Manley, R. R. Dasari, K. Badizadegan, and M. S. Feld, “Optical measurement of cell membrane tension,” Phys. Rev. Lett. 97(21), 218101 (2006). [CrossRef] [PubMed]
  7. X. Li, T. Yamauchi, H. Iwai, Y. Yamashita, H. Zhang, and T. Hiruma, “Full-field quantitative phase imaging by white-light interferometry with active phase stabilization and its application to biological samples,” Opt. Lett. 31(12), 1830–1832 (2006). [CrossRef] [PubMed]
  8. P. Marquet, B. Rappaz, P. J. Magistretti, E. Cuche, Y. Emery, T. Colomb, and C. Depeursinge, “Digital holographic microscopy: a noninvasive contrast imaging technique allowing quantitative visualization of living cells with subwavelength axial accuracy,” Opt. Lett. 30(5), 468–470 (2005). [CrossRef] [PubMed]
  9. B. Rappaz, P. Marquet, E. Cuche, Y. Emery, C. Depeursinge, and P. Magistretti, “Measurement of the integral refractive index and dynamic cell morphometry of living cells with digital holographic microscopy,” Opt. Express 13(23), 9361–9373 (2005). [CrossRef] [PubMed]
  10. W. Drexler, U. Morgner, F. X. Kärtner, C. Pitris, S. A. Boppart, X. D. Li, E. P. Ippen, and J. G. Fujimoto, “In vivo ultrahigh-resolution optical coherence tomography,” Opt. Lett. 24(17), 1221–1223 (1999). [CrossRef]
  11. S. Makita, T. Fabritius, and Y. Yasuno, “Full-range, high-speed, high-resolution 1 microm spectral-domain optical coherence tomography using BM-scan for volumetric imaging of the human posterior eye,” Opt. Express 16(12), 8406–8420 (2008). [CrossRef] [PubMed]
  12. A. Dubois, K. Grieve, G. Moneron, R. Lecaque, L. Vabre, and C. Boccara, “Ultrahigh-resolution full-field optical coherence tomography,” Appl. Opt. 43(14), 2874–2883 (2004). [CrossRef] [PubMed]
  13. C. Yang, A. Wax, M. S. Hahn, K. Badizadegan, R. R. Dasari, and M. S. Feld, “Phase-referenced interferometer with subwavelength and subhertz sensitivity applied to the study of cell membrane dynamics,” Opt. Lett. 26(16), 1271–1273 (2001). [CrossRef]
  14. J. Kühn, T. Colomb, F. Montfort, F. Charrière, Y. Emery, E. Cuche, P. Marquet, and C. Depeursinge, “Real-time dual-wavelength digital holographic microscopy with a single hologram acquisition,” Opt. Express 15(12), 7231–7242 (2007). [CrossRef] [PubMed]
  15. C. Fang-Yen, M. C. Chu, H. S. Seung, R. R. Dasari, and M. S. Feld, “Noncontact measurement of nerve displacement during action potential with a dual-beam low-coherence interferometer,” Opt. Lett. 29(17), 2028–2030 (2004). [CrossRef] [PubMed]
  16. T. Akkin, D. Davé, T. Milner, and H. Rylander Iii, “Detection of neural activity using phase-sensitive optical low-coherence reflectometry,” Opt. Express 12(11), 2377–2386 (2004). [CrossRef] [PubMed]
  17. K. Jeong, J. J. Turek, and D. D. Nolte, “Fourier-domain digital holographic optical coherence imaging of living tissue,” Appl. Opt. 46(22), 4999–5008 (2007). [CrossRef] [PubMed]
  18. K. Jeong, J. J. Turek, and D. D. Nolte, “Volumetric motility-contrast imaging of tissue response to cytoskeletal anti-cancer drugs,” Opt. Express 15(21), 14057–14064 (2007). [CrossRef] [PubMed]
  19. A. K. Ellerbee, T. L. Creazzo, and J. A. Izatt, “Investigating nanoscale cellular dynamics with cross-sectional spectral domain phase microscopy,” Opt. Express 15(13), 8115–8124 (2007). [CrossRef] [PubMed]
  20. D. C. Adler, R. Huber, and J. G. Fujimoto, “Phase-sensitive optical coherence tomography at up to 370,000 lines per second using buffered Fourier domain mode-locked lasers,” Opt. Lett. 32(6), 626–628 (2007). [CrossRef] [PubMed]
  21. D. C. Adler, S. W. Huang, R. Huber, and J. G. Fujimoto, “Photothermal detection of gold nanoparticles using phase-sensitive optical coherence tomography,” Opt. Express 16(7), 4376–4393 (2008). [CrossRef] [PubMed]
  22. J. Oh, M. D. Feldman, J. Kim, H. W. Kang, P. Sanghi, and T. E. Milner, “Magneto-motive detection of tissue-based macrophages by differential phase optical coherence tomography,” Lasers Surg. Med. 39(3), 266–272 (2007). [CrossRef] [PubMed]
  23. T. Yamauchi, H. Iwai, M. Miwa, and Y. Yamashita, “Low-coherent quantitative phase microscope for nanometer-scale measurement of living cells morphology,” Opt. Express 16(16), 12227–12238 (2008). [CrossRef] [PubMed]
  24. A. Dubois, J. Selb, L. Vabre, and A. C. Boccara, “Phase measurements with wide-aperture interferometers,” Appl. Opt. 39(14), 2326–2331 (2000). [CrossRef]
  25. K. Creath, “V Phase-Measurement Interferometry Techniques,” in Progress in Optics, E. Wolf, ed. (Elsevier, 1988), pp. 349–393.
  26. K. Hibino, B. F. Oreb, D. I. Farrant, and K. G. Larkin, “Phase shifting for nonsinusoidal waveforms with phase-shift errors,” J. Opt. Soc. Am. A 12(4), 761–768 (1995). [CrossRef]
  27. M. H. Gail and C. W. Boone, “The locomotion of mouse fibroblasts in tissue culture,” Biophys. J. 10(10), 980–993 (1970). [CrossRef] [PubMed]
  28. H. Qian, M. P. Sheetz, and E. L. Elson, “Single particle tracking. Analysis of diffusion and flow in two-dimensional systems,” Biophys. J. 60(4), 910–921 (1991). [CrossRef] [PubMed]
  29. G. Popescu, Y. Park, N. Lue, C. Best-Popescu, L. Deflores, R. R. Dasari, M. S. Feld, and K. Badizadegan, “Optical imaging of cell mass and growth dynamics,” Am. J. Physiol. Cell Physiol. 295(2), C538–C544 (2008). [CrossRef] [PubMed]
  30. N. T. Shaked, L. L. Satterwhite, N. Bursac, and A. Wax, “Whole-cell-analysis of live cardiomyocytes using wide-field interferometric phase microscopy,” Biomed Opt. Express 1(2), 706–719 (2010). [CrossRef]
  31. J. A. Hessler, A. Budor, K. Putchakayala, A. Mecke, D. Rieger, M. M. Banaszak Holl, B. G. Orr, A. Bielinska, J. Beals, and J. Baker., “Atomic force microscopy study of early morphological changes during apoptosis,” Langmuir 21(20), 9280–9286 (2005). [CrossRef] [PubMed]
  32. C. P. Brophy, “Effect of intensity error correlation on the computed phase of phase-shifting interferometry,” J. Opt. Soc. Am. A 7(4), 537–541 (1990). [CrossRef]

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