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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 6, Iss. 4 — May. 4, 2011

Spectral characterization of biological aerosol particles using two-wavelength excited laser-induced fluorescence and elastic scattering measurements

Vasanthi Sivaprakasam, Horn-Bond Lin, Alan L. Huston, and Jay D. Eversole  »View Author Affiliations

Optics Express, Vol. 19, Issue 7, pp. 6191-6208 (2011)

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A two-wavelength laser-induced fluorescence (LIF) instrument has been developed and used to characterize individual biological aerosol particles, including biological warfare (BW) agent surrogates. Fluorescence in discrete spectral bands from widely different species, and also from similar species under different growth conditions were measured and compared. The two-wavelength excitation approach was found to increase discrimination among several biological materials, and especially with respect to diesel exhaust particles, a common interferent for LIF BW detection systems. The spectral characteristics of a variety of biological materials and ambient air components have been studied as a function of aerosol particle size and incident fluence.

© 2011 OSA

OCIS Codes
(010.1100) Atmospheric and oceanic optics : Aerosol detection
(290.5850) Scattering : Scattering, particles
(300.2530) Spectroscopy : Fluorescence, laser-induced

ToC Category:
Atmospheric and Oceanic Optics

Original Manuscript: December 2, 2010
Revised Manuscript: February 3, 2011
Manuscript Accepted: February 4, 2011
Published: March 18, 2011

Virtual Issues
Vol. 6, Iss. 4 Virtual Journal for Biomedical Optics

Vasanthi Sivaprakasam, Horn-Bond Lin, Alan L. Huston, and Jay D. Eversole, "Spectral characterization of biological aerosol particles using two-wavelength excited laser-induced fluorescence and elastic scattering measurements," Opt. Express 19, 6191-6208 (2011)

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