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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 7, Iss. 3 — Feb. 29, 2012

Correcting the detrimental effects of nonuniform intensity distribution on fiber-transmitting laser speckle imaging of blood flow

Hongyan Zhang, Pengcheng Li, Nengyun Feng, Jianjun Qiu, Bing Li, Weihua Luo, and Qingming Luo  »View Author Affiliations

Optics Express, Vol. 20, Issue 1, pp. 508-517 (2012)

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Laser speckle spatial contrast analysis (LSSCA) is superior to laser speckle temporal contrast analysis (LSTCA) in monitoring the fast change in blood flow due to its advantage of high temporal resolution. However, the application of LSSCA which is based on spatial statistics may be limited when there is nonuniform intensity distribution such as fiber-transmitting laser speckle imaging. In this study, we present a normalized laser speckle spatial contrast analysis (nLSSCA) to correct the detrimental effects of nonuniform intensity distribution on the spatial statistics. Through numerical simulation and phantom experiments, it is found that just ten frames of dynamic laser speckle images are sufficient for nLSSCA to achieve effective correction. Furthermore, nLSSCA has higher temporal resolution than LSTCA to respond the change in velocity. LSSCA, LSTCA and nLSSCA are all applied in the fiber-transmitting laser speckle imaging system to analyze the change of cortical blood flow (CBF) during cortical spreading depression (CSD) in rat cortex respectively, and the results suggest that nLSSCA can examine the change of CBF more accurately. For these advantages, nLSSCA could be a potential tool for fiber-transmitting/endoscopic laser speckle imaging.

© 2011 OSA

OCIS Codes
(060.2350) Fiber optics and optical communications : Fiber optics imaging
(100.2960) Image processing : Image analysis
(110.6150) Imaging systems : Speckle imaging

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: September 26, 2011
Revised Manuscript: November 23, 2011
Manuscript Accepted: December 11, 2011
Published: December 21, 2011

Virtual Issues
Vol. 7, Iss. 3 Virtual Journal for Biomedical Optics

Hongyan Zhang, Pengcheng Li, Nengyun Feng, Jianjun Qiu, Bing Li, Weihua Luo, and Qingming Luo, "Correcting the detrimental effects of nonuniform intensity distribution on fiber-transmitting laser speckle imaging of blood flow," Opt. Express 20, 508-517 (2012)

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