OSA's Digital Library

Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 7, Iss. 7 — Jun. 25, 2012

Digital holographic microscopy long-term and real-time monitoring of cell division and changes under simulated zero gravity

Feng Pan, Shuo Liu, Zhe Wang, Peng Shang, and Wen Xiao  »View Author Affiliations

Optics Express, Vol. 20, Issue 10, pp. 11496-11505 (2012)

View Full Text Article

Enhanced HTML    Acrobat PDF (1231 KB) Open Access

Browse Journals / Lookup Meetings

Browse by Journal and Year


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools



The long-term and real-time monitoring the cell division and changes of osteoblasts under simulated zero gravity condition were succeed by combing a digital holographic microscopy (DHM) with a superconducting magnet (SM). The SM could generate different magnetic force fields in a cylindrical cavity, where the gravitational force of biological samples could be canceled at a special gravity position by a high magnetic force. Therefore the specimens were levitated and in a simulated zero gravity environment. The DHM was modified to fit with SM by using single mode optical fibers and a vertically-configured jig designed to hold specimens and integrate optical device in the magnet’s bore. The results presented the first-phase images of living cells undergoing dynamic divisions and changes under simulated zero gravity environment for a period of 10 hours. The experiments demonstrated that the SM-compatible DHM setup could provide a highly efficient and versatile method for research on the effects of microgravity on biological samples.

© 2012 OSA

OCIS Codes
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(180.0180) Microscopy : Microscopy
(090.1995) Holography : Digital holography

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: March 6, 2012
Revised Manuscript: April 27, 2012
Manuscript Accepted: May 1, 2012
Published: May 4, 2012

Virtual Issues
Vol. 7, Iss. 7 Virtual Journal for Biomedical Optics

Feng Pan, Shuo Liu, Zhe Wang, Peng Shang, and Wen Xiao, "Digital holographic microscopy long-term and real-time monitoring of cell division and changes under simulated zero gravity," Opt. Express 20, 11496-11505 (2012)

Sort:  Author  |  Year  |  Journal  |  Reset  


  1. R. J. White, “Weightlessness and the human body,” Sci. Am.279(3), 58–63 (1998). [CrossRef] [PubMed]
  2. R. J. White and M. Averner, “Humans in space,” Nature409(6823), 1115–1118 (2001). [CrossRef] [PubMed]
  3. A. Geim, “Everyone’s magnetism,” Phys. Today51(9), 36–39 (1998). [CrossRef]
  4. E. Beaugnon and R. Tournier, “Levitation of organic materials,” Nature349(6309), 470 (1991). [CrossRef]
  5. K. Guevorkian and J. M. Valles., “Swimming Paramecium in magnetically simulated enhanced, reduced, and inverted gravity environments,” Proc. Natl. Acad. Sci. U.S.A.103(35), 13051–13056 (2006). [CrossRef] [PubMed]
  6. A. R. Qian, D. C. Yin, P. F. Yang, B. Jia, W. Zhang, and P. Shang, “Development of a Ground-based Simulated Experimental Platform for Gravitational Biology,” IEEE Trans. Appl. Supercon.19(2), 42–46 (2009). [CrossRef]
  7. B. E. Hammer, L. S. Kidder, P. C. Williams, and W. W. Xu, “Magnetic levitation of MC3T3 osteoblast cells as a ground-based simulation of microgravity,” Microgravity Sci. Technol.21(4), 311–318 (2009). [CrossRef] [PubMed]
  8. A. R. Qian, W. Zhang, Y. Y. Weng, Z. C. Tian, S. M. Di, P. F. Yang, D. C. Yin, L. F. Hu, Z. Wang, H. Y. Xu, and P. Shang, “Gravitational environment produced by a superconducting magnet affects osteoblast morphology and functions,” Acta Astronaut.63(7-10), 929–946 (2008). [CrossRef]
  9. A. R. Qian, L. Wang, X. Gao, W. Zhang, L. F. Hu, J. Han, J. B. Li, S. M. Di, and P. Shang, “Diamagnetic levitation causes changes in the morphology, cytoskeleton, and focal adhesion proteins expression in osteocytes,” IEEE Trans. Biomed. Eng.59(1), 68–77 (2012). [CrossRef] [PubMed]
  10. P. Marquet, B. Rappaz, P. J. Magistretti, E. Cuche, Y. Emery, T. Colomb, and C. Depeursinge, “Digital holographic microscopy: a noninvasive contrast imaging technique allowing quantitative visualization of living cells with subwavelength axial accuracy,” Opt. Lett.30(5), 468–470 (2005). [CrossRef] [PubMed]
  11. B. Kemper and G. von Bally, “Digital holographic microscopy for live cell applications and technical inspection,” Appl. Opt.47(4), A52–A61 (2008). [CrossRef] [PubMed]
  12. B. Kemper, A. Bauwens, A. Vollmer, S. Ketelhut, P. Langehanenberg, J. Müthing, H. Karch, and G. von Bally, “Label-free quantitative cell division monitoring of endothelial cells by digital holographic microscopy,” J. Biomed. Opt.15(3), 036009 (2010). [CrossRef] [PubMed]
  13. C. J. Mann, L. Y. Yu, and M. K. Kim, “Movies of cellular and sub-cellular motion by digital holographic microscopy,” Biomed. Eng. Online5(1), 21 (2006). [CrossRef] [PubMed]
  14. C. Pache, J. Kühn, K. Westphal, M. F. Toy, J. M. Parent, O. Büchi, A. Franco-Obregón, C. Depeursinge, and M. Egli, “Digital holographic microscopy real-time monitoring of cytoarchitectural alterations during simulated microgravity,” J. Biomed. Opt.15(2), 026021 (2010). [CrossRef] [PubMed]
  15. M. F. Toy, S. Richard, J. Kühn, A. Franco-Obregón, M. Egli, and C. Depeursinge, “Enhanced robustness digital holographic microscopy for demanding environment of space biology,” Biomed. Opt. Express3(2), 313–326 (2012). [CrossRef] [PubMed]
  16. E. Cuche, P. Marquet, and C. Depeursinge, “Aperture apodization using cubic spline interpolation: application in digital holographic microscopy,” Opt. Commun.182(1-3), 59–69 (2000). [CrossRef]
  17. E. Cuche, P. Marquet, and C. Depeursinge, “Spatial filtering for zero-order and twin-image elimination in digital off-axis holography,” Appl. Opt.39(23), 4070–4075 (2000). [CrossRef] [PubMed]
  18. T. Colomb, E. Cuche, F. Charrière, J. Kühn, N. Aspert, F. Montfort, P. Marquet, and C. Depeursinge, “Automatic procedure for aberration compensation in digital holographic microscopy and applications to specimen shape compensation,” Appl. Opt.45(5), 851–863 (2006). [CrossRef] [PubMed]
  19. B. Rappaz, P. Marquet, E. Cuche, Y. Emery, C. Depeursinge, and P. J. Magistretti, “Measurement of the integral refractive index and dynamic cell morphometry of living cells with digital holographic microscopy,” Opt. Express13(23), 9361–9373 (2005), http://www.opticsinfobase.org/oe/abstract.cfm?uri=oe-13-23-9361 . [CrossRef] [PubMed]
  20. B. Kemper, D. Carl, J. Schnekenburger, I. Bredebusch, M. Schäfer, W. Domschke, and G. von Bally, “Investigation of living pancreas tumor cells by digital holographic microscopy,” J. Biomed. Opt.11(3), 034005 (2006). [CrossRef] [PubMed]
  21. B. Rappaz, F. Charrière, C. Depeursinge, P. J. Magistretti, and P. Marquet, “Simultaneous cell morphometry and refractive index measurement with dual-wavelength digital holographic microscopy and dye-enhanced dispersion of perfusion medium,” Opt. Lett.33(7), 744–746 (2008). [CrossRef] [PubMed]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.

Supplementary Material

» Media 1: MOV (2397 KB)     

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited