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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 7, Iss. 8 — Aug. 2, 2012

The noise-limited-resolution for stimulated emission depletion microscopy of diffusing particles

Chris J. Lee and Klaus J. Boller  »View Author Affiliations

Optics Express, Vol. 20, Issue 12, pp. 12793-12798 (2012)

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With recent developments in microscopy, such as stimulated emission depletion (STED) microscopy, far-field imaging at resolutions better than the diffraction limit is now a commercially available technique. Here, we show that, in the special case of a diffusive regime, the noise-limited resolution of STED imaging is independent of the saturation intensity of the fluorescent label. Thermal motion limits the signal integration time, which, for a given excited-state lifetime, limits the total number of photons available for detection.

© 2012 OSA

OCIS Codes
(110.0110) Imaging systems : Imaging systems
(110.0180) Imaging systems : Microscopy
(110.4280) Imaging systems : Noise in imaging systems
(180.2520) Microscopy : Fluorescence microscopy

ToC Category:

Original Manuscript: February 28, 2012
Revised Manuscript: April 19, 2012
Manuscript Accepted: April 24, 2012
Published: May 23, 2012

Virtual Issues
Vol. 7, Iss. 8 Virtual Journal for Biomedical Optics

Chris J. Lee and Klaus J. Boller, "The noise-limited-resolution for stimulated emission depletion microscopy of diffusing particles," Opt. Express 20, 12793-12798 (2012)

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