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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 7, Iss. 8 — Aug. 2, 2012

New sub-diffraction-limit microscopy technique: Dual-point illumination AND-gate microscopy on nanodiamonds (DIAMOND)

Jiwoong Kwon, Youngbin Lim, Jiwon Jung, and Seong Keun Kim  »View Author Affiliations


Optics Express, Vol. 20, Issue 12, pp. 13347-13356 (2012)
http://dx.doi.org/10.1364/OE.20.013347


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Abstract

We introduce a new, easily implementable sub-diffraction-limit microscopy technique utilizing the optical AND-gate property of fluorescent nanodiamond (FND). We demonstrate that when FND is illuminated by two spatially-offset lights of different wavelengths, emission comes only from the region of their overlap, which is used to reduce the effective point spread function from ~300 nm to ~130 nm in lateral plane, well below the diffraction limit.

© 2012 OSA

OCIS Codes
(180.1790) Microscopy : Confocal microscopy
(180.2520) Microscopy : Fluorescence microscopy
(350.5730) Other areas of optics : Resolution

ToC Category:
Microscopy

History
Original Manuscript: April 9, 2012
Revised Manuscript: May 22, 2012
Manuscript Accepted: May 22, 2012
Published: May 30, 2012

Virtual Issues
Vol. 7, Iss. 8 Virtual Journal for Biomedical Optics

Citation
Jiwoong Kwon, Youngbin Lim, Jiwon Jung, and Seong Keun Kim, "New sub-diffraction-limit microscopy technique: Dual-point illumination AND-gate microscopy on nanodiamonds (DIAMOND)," Opt. Express 20, 13347-13356 (2012)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=oe-20-12-13347


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