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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 7, Iss. 8 — Aug. 2, 2012

Polarization-resolved second harmonic generation microscopy with a four-channel Stokes-polarimeter

Nirmal Mazumder, Jianjun Qiu, Matthew R. Foreman, Carlos Macías Romero, Chih-Wei Hu, Han-Ruei Tsai, Peter Török, and Fu-Jen Kao  »View Author Affiliations

Optics Express, Vol. 20, Issue 13, pp. 14090-14099 (2012)

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We developed a four-channel photon counting based Stokes-polarimeter for spatial characterization of polarization effects in second harmonic generation (SHG). We have implemented a calibration technique allowing quantitative measurement of polarization parameters, such as the degree of polarization (DOP), degree of linear polarization (DOLP), degree of circular polarization (DOCP), as well as anisotropy from the acquired Stokes parameters. The technique is used as contrast mechanism to characterize the polarization properties from two potassium dihydrogen phosphate (KDP) micro-crystals and collagen type-I in SHG microscopy.

© 2012 OSA

OCIS Codes
(120.0120) Instrumentation, measurement, and metrology : Instrumentation, measurement, and metrology
(120.5410) Instrumentation, measurement, and metrology : Polarimetry
(320.0320) Ultrafast optics : Ultrafast optics
(180.4315) Microscopy : Nonlinear microscopy

ToC Category:

Original Manuscript: April 27, 2012
Revised Manuscript: June 1, 2012
Manuscript Accepted: June 1, 2012
Published: June 11, 2012

Virtual Issues
Vol. 7, Iss. 8 Virtual Journal for Biomedical Optics

Nirmal Mazumder, Jianjun Qiu, Matthew R. Foreman, Carlos Macías Romero, Chih-Wei Hu, Han-Ruei Tsai, Peter Török, and Fu-Jen Kao, "Polarization-resolved second harmonic generation microscopy with a four-channel Stokes-polarimeter," Opt. Express 20, 14090-14099 (2012)

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