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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 7, Iss. 8 — Aug. 2, 2012

Quantitative 3D imaging of scattering media using structured illumination and computed tomography

E. Kristensson, E. Berrocal, and M. Aldén  »View Author Affiliations

Optics Express, Vol. 20, Issue 13, pp. 14437-14450 (2012)

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An imaging technique capable of measuring the extinction coefficient in 3D is presented and demonstrated on various scattering media. The approach is able to suppress unwanted effects due to both multiple scattering and light extinction, which, in turbid situations, seriously hampers the performance of conventional imaging techniques. The main concept consists in illuminating the sample of interest with a light source that is spatially modulated in both the vertical and horizontal direction and to measure, using Structured Illumination, the correct transmission in 2D at several viewing angles. The sample is then reconstructed in 3D by means of a standard Computed Tomography algorithm. To create the adequate illumination, a novel “crossed” structured illumination approach is implemented. In this article, the accuracy and limitation of the method is first evaluated by probing several homogeneous milk solutions at various levels of turbidity. The unique possibility of visualizing an object hidden within such solutions is also demonstrated. Finally the method is applied on two different inhomogeneous scattering spray systems; one transient and one quasi-steady state.

© 2012 OSA

OCIS Codes
(290.2200) Scattering : Extinction
(290.4210) Scattering : Multiple scattering
(290.7050) Scattering : Turbid media
(110.0113) Imaging systems : Imaging through turbid media
(110.6955) Imaging systems : Tomographic imaging

ToC Category:
Imaging Systems

Original Manuscript: February 1, 2012
Revised Manuscript: March 29, 2012
Manuscript Accepted: April 11, 2012
Published: June 13, 2012

Virtual Issues
Vol. 7, Iss. 8 Virtual Journal for Biomedical Optics

E. Kristensson, E. Berrocal, and M. Aldén, "Quantitative 3D imaging of scattering media using structured illumination and computed tomography," Opt. Express 20, 14437-14450 (2012)

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  1. M. Padgett, “Penetrating scattering media,” Nat. Photonics4, 741–742 (2010). [CrossRef]
  2. M. Linne, M. Paciaroni, E. Berrocal, and D. Sedarsky, “Ballistic imaging of liquid breakup processes in dense sprays,” in Proceedings of the Combustion Institute (2009), Vol. 32, pp. 2147–2161. [CrossRef]
  3. O. S. Ugolnikov, O. V. Postylyakov, and I. A. Maslova, “Effects of multiple scattering and atmospheric aerosol on the polarization of the twilight sky,” J. Quant. Spectrosc. Radiat. Transfer88, 233–241 (2004). [CrossRef]
  4. S. G. Narasimhan, S. K. Nayar, B. Sun, and S. J. Koppal, “Structured light in scattering media,” in Proceedings of the Tenth IEEE International Conference on Computer Vision (2005).
  5. G. Zaccanti, P. Bruscaglioni, M. Gurioli, and P. Sansoni, “Laboratory simulations of lidar returns from clouds: experimental and numerical results,” Appl. Opt.32, 1590–1597 (1993). [CrossRef] [PubMed]
  6. W.-F. Cheong, S. A. Prahl, and A. J. Welch, “A review of the optical properties of biological tissues,” IEEE J. Quant. Electron.26, 2166–2185 (1990). [CrossRef]
  7. T. Breuninger, K. Greger, and E. H. K. Stelzer, “Lateral modulation boosts image quality in single plane illumination fluorescence microscopy,” Opt. Lett.32, 1938–1940 (2007). [CrossRef] [PubMed]
  8. E. Berrocal, E. Kristensson, M. Richter, M. Linne, and M. Aldén, “Application of structured illumination for multiple scattering suppression in planar laser imaging of dense sprays,” Opt. Express16, 17870–17881 (2008). [CrossRef] [PubMed]
  9. R. Wellander, E. Berrocal, E. Kristensson, M. Richter, and M. Aldén, “Three-dimensional measurement of the local extinction coefficient in a dense spray,” Meas. Sci. Technol.22, 855–861 (2011). [CrossRef]
  10. C. F. Powell, Y. Yue, R. Poolab, and J. Wanga, “Time-resolved measurements of supersonic fuel sprays using synchrotron x-rays,” J. Synchrotron Radiat.7, 356–360 (2000). [CrossRef]
  11. W. Cai, C. F. Powell, Y. Yue, S. Narayanan, J. Wanga, M. W. Tate, M. J. Renzi, A. Ercan, E. Fontes, and S. M. Grunere, “Quantitative analysis of highly transient fuel sprays by time-resolved x-radiography,” Appl. Phys. Lett.83, 1671–1673 (2003). [CrossRef]
  12. A. Kak and M. Slaney, Principles of Computerized Tomographic Imaging (Academic Press, 1999).
  13. M. A. A. Neil, R. Juškaitis, and T. Wilson, “Method of obtaining optical sectioning by using structured light in a conventional microscope,” Opt. Lett.22, 1905–1907 (1997). [CrossRef]
  14. Q. Wu, F. A. Merchant, and K. R. Castleman, Microscope Image Processing (Academic Press, 2008).
  15. D. J. Cuccia, F. Bevilacqua, A. J. Durkin, and B. J. Tromberg, “Modulated imaging: quantitative analysis and tomography of turbid media in the spatial-frequency domain,” Opt. Lett.30, 1354–1356 (2005). [CrossRef] [PubMed]
  16. J. Chen, V. Venugopal, F. Lesage, and X. Intes, “Time-resolved diffuse optical tomography with patterned-light illumination and detection,” Opt. Lett.35, 2121–2123 (2010). [CrossRef] [PubMed]
  17. N. Ducros, A. Bassi, G. Valentini, M. Schweiger, S. Arridge, and C. D’Andrea, “Muliple-view fluorescence optical tomography reconstruction using compression of experimental data,” Opt. Lett.36, 1377–1379 (2011). [CrossRef] [PubMed]
  18. W. Choi, C. Fang-Yen, K. Badizadegan, S. Oh, N. Lue, R. R. Dasari, and M. S. Feld, “Tomographic phase microscopy,” Nat. Methods4, 717–719 (2007). [CrossRef] [PubMed]
  19. E. Berrocal, D. L. Sedarsky, M. E. Paciaroni, I. V. Meglinski, and M. A. Linne, “Laser light scattering in turbid media. Part I: Experimental and simulated results for the spatial intensity distribution,” Opt. Express15, 10649–10665 (2007). [CrossRef] [PubMed]
  20. E. Kristensson, L. Araneo, E. Berrocal, J. Manin, M. Richter, M. Aldén, and M. Linne, “Analysis of multiple scattering suppression using structured laser illumination planar imaging in scattering and fluorescing media,” Opt. Express19, 13674–13663 (2011). [CrossRef]

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