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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 7, Iss. 9 — Aug. 28, 2012

SLM-based off-axis Fourier filtering in microscopy with white light illumination

Ruth Steiger, Stefan Bernet, and Monika Ritsch-Marte  »View Author Affiliations


Optics Express, Vol. 20, Issue 14, pp. 15377-15384 (2012)
http://dx.doi.org/10.1364/OE.20.015377


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Abstract

In various microscopy applications spatial light modulators (SLMs) are used as programmable Fourier filters to realize different optical contrast enhancement methods. It is often advantageous to use the SLM in off-axis configuration, where the filtered image wave is sent into the first diffraction order of a blazed grating superposed to the phase mask on the SLM. Because of dispersion this approach is, however, typically limited to spectrally narrowband illumination. Here we suggest a method involving a grating for pre-compensation, which allows one to use spectrally broadband (even thermal) light in SLM-based Fourier filtering. The proposed approach is demonstrated by multicolor imaging of amplitude and phase objects, such as a resolution target, onion epidermal cells and human epithelial cheek cells.

© 2012 OSA

OCIS Codes
(070.6110) Fourier optics and signal processing : Spatial filtering
(170.0180) Medical optics and biotechnology : Microscopy
(230.1950) Optical devices : Diffraction gratings
(230.3720) Optical devices : Liquid-crystal devices
(260.2030) Physical optics : Dispersion

ToC Category:
Microscopy

History
Original Manuscript: April 24, 2012
Revised Manuscript: June 15, 2012
Manuscript Accepted: June 20, 2012
Published: June 25, 2012

Virtual Issues
Vol. 7, Iss. 9 Virtual Journal for Biomedical Optics

Citation
Ruth Steiger, Stefan Bernet, and Monika Ritsch-Marte, "SLM-based off-axis Fourier filtering in microscopy with white light illumination," Opt. Express 20, 15377-15384 (2012)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=oe-20-14-15377


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References

  1. C. Maurer, A. Jesacher, S. Bernet, and M. Ritsch-Marte, “What spatial light modulators can do for optical microscopy,” Laser Photonics Rev.5(1), 81–101 (2011). [CrossRef]
  2. J. A. Neff, R. A. Athale, and S. H. Lee, “Two-dimensional spatial light modulators: A tutorial,” Proc. IEEE78(5), 826–855 (1990). [CrossRef]
  3. F. Yaras, H. Kang, and L. Onural, “State of the Art in Holographic Displays: A Survey,” J.Disp. Technol.6(10), 443–454 (2010). [CrossRef]
  4. M. Warber, S. Zwick, M. Hasler, T. Haist, and W. Osten, “SLM-based phase-contrast filtering for single and multiple image acquisition,” Proc. SPIE7442, 74420E (2009). [CrossRef]
  5. S. Fürhapter, A. Jesacher, S. Bernet, and M. Ritsch-Marte, “Spiral phase contrast imaging in microscopy,” Opt. Express13(3), 689–694 (2005). [CrossRef] [PubMed]
  6. K. D. Wulff, D. G. Cole, R. L. Clark, R. Dileonardo, J. Leach, J. Cooper, G. Gibson, and M. J. Padgett, “Aberration correction in holographic optical tweezers,” Opt. Express14(9), 4170–4175 (2006). [CrossRef] [PubMed]
  7. D. G. Grier, “A revolution in optical manipulation,” Nature424(6950), 810–816 (2003). [CrossRef] [PubMed]
  8. K. Dholakia and T. Čižmár, “Shaping the future of manipulation,” Nat. Photonics5(6), 335–342 (2011). [CrossRef]
  9. J. Glückstad, “Phase contrast image synthesis,” Opt. Commun.130(4-6), 225–230 (1996). [CrossRef]
  10. M. Woerdemann, F. Holtmann, and C. Denz, “Holographic phase contrast for dynamic multiple-beam optical tweezers,” J. Opt. A, Pure Appl. Opt.11(3), 034010 (2009). [CrossRef]
  11. C.-S. Guo, Y.-J. Han, J.-B. Xu, and J. Ding, “Radial Hilbert transform with Laguerre-Gaussian spatial filters,” Opt. Lett.31(10), 1394–1396 (2006). [CrossRef] [PubMed]
  12. P. Birch, R. Young, C. Chatwin, M. Farsari, D. Budgett, and J. Richardson, “Fully complex optical modulation with an analogue ferroelectric liquid crystal spatial light modulator,” Opt. Commun.175(4-6), 347–352 (2000). [CrossRef]
  13. S. E. Schausberger, B. Heise, C. Maurer, S. Bernet, M. Ritsch-Marte, and D. Stifter, “Flexible contrast for low-coherence interference microscopy by Fourier-plane filtering with a spatial light modulator,” Opt. Lett.35(24), 4154–4156 (2010). [CrossRef] [PubMed]
  14. J. Leach and M. J. Padgett, “Observation of chromatic effects near a white-light vortex,” New J. Phys.5, 154–160 (2003). [CrossRef]
  15. S. Bernet, A. Jesacher, S. Fürhapter, C. Maurer, and M. Ritsch-Marte, “Quantitative imaging of complex samples by spiral phase contrast microscopy,” Opt. Express14(9), 3792–3805 (2006). [CrossRef] [PubMed]
  16. H. Ding and G. Popescu, “Instantaneous spatial light interference microscopy,” Opt. Express18(2), 1569–1575 (2010). [CrossRef] [PubMed]
  17. B. Bhaduri, H. Pham, M. Mir, and G. Popescu, “Diffraction phase microscopy with white light,” Opt. Lett.37(6), 1094–1096 (2012). [CrossRef] [PubMed]
  18. C. Kohler, X. Schwab, and W. Osten, “Optimally tuned spatial light modulators for digital holography,” Appl. Opt.45(5), 960–967 (2006). [CrossRef] [PubMed]
  19. T. Ito and K. Okano, “Color electroholography by three colored reference lights simultaneously incident upon one hologram panel,” Opt. Express12(18), 4320–4325 (2004). [CrossRef] [PubMed]

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