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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 7, Iss. 9 — Aug. 28, 2012

Phase contrast microscopy with fringe contrast adjustable by using grating-based phase-shifter

Juanjuan Zheng, Baoli Yao, Peng Gao, and Tong Ye  »View Author Affiliations

Optics Express, Vol. 20, Issue 14, pp. 16077-16082 (2012)

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In this paper a new phase contrast method with fringe contrast adjustable is proposed. In the Fourier plane of the object wave, two Ronchi gratings i.e., a central grating and a surrounding grating, are used to modulate the phases of the undiffracted and diffracted components, respectively. By loading the two gratings separately on spatial light modulator, the undiffracted and diffracted components can be measured independently, which simplify greatly the reconstruction process. Besides, the fringe contrast of the phase contrast interferogram can be adjusted by changing the modulation depth of the two gratings. The feasibility of the proposed method is verified by theoretical analysis and experiment.

© 2012 OSA

OCIS Codes
(070.6110) Fourier optics and signal processing : Spatial filtering
(100.5090) Image processing : Phase-only filters
(170.0180) Medical optics and biotechnology : Microscopy

ToC Category:

Original Manuscript: May 10, 2012
Revised Manuscript: June 22, 2012
Manuscript Accepted: June 23, 2012
Published: June 29, 2012

Virtual Issues
Vol. 7, Iss. 9 Virtual Journal for Biomedical Optics

Juanjuan Zheng, Baoli Yao, Peng Gao, and Tong Ye, "Phase contrast microscopy with fringe contrast adjustable by using grating-based phase-shifter," Opt. Express 20, 16077-16082 (2012)

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  1. G. Popescu, T. Ikeda, R. R. Dasari, and M. S. Feld, “Diffraction phase microscopy for quantifying cell structure and dynamics,” Opt. Lett.31(6), 775–777 (2006). [CrossRef] [PubMed]
  2. B. Bhaduri, H. Pham, M. Mir, and G. Popescu, “Diffraction phase microscopy with white light,” Opt. Lett.37(6), 1094–1096 (2012). [CrossRef] [PubMed]
  3. V. Mico, Z. Zalevsky, and J. García, “Superresolution optical system by common-path interferometry,” Opt. Express14(12), 5168–5177 (2006). [CrossRef] [PubMed]
  4. P. Gao, I. Harder, V. Nercissian, K. Mantel, and B. Yao, “Phase-shifting point-diffraction interferometry with common-path and in-line configuration for microscopy,” Opt. Lett.35(5), 712–714 (2010). [CrossRef] [PubMed]
  5. G. Popescu, Y. K. Park, K. Badizadegan, R. R. Dasari, and M. S. Feld, “Diffraction phase and fluorescence microscopy,” Opt. Express14(18), 8263–8268 (2006). [CrossRef] [PubMed]
  6. F. Zernike, “Phase contrast, a new method for the microscopic observation of transparent objects,” Physica9(7Part I), 686–698 (1942). [CrossRef]
  7. R. Liang, J. K. Erwin, and M. Mansuripur, “Variation on Zernike’s phase-contrast microscope,” Appl. Opt.39(13), 2152–2158 (2000). [CrossRef] [PubMed]
  8. Z. Wang, L. Millet, M. Mir, H. Ding, S. Unarunotai, J. Rogers, M.-U. Gillette, and G. Popescu, “Spatial light interference microscopy (SLIM),” Opt. Express19(2), 1016–1026 (2011). [CrossRef] [PubMed]
  9. C. Maurer, A. Jesacher, S. Bernet, and M. Ritsch-Marte, “Phase contrast microscopy with full numerical aperture illumination,” Opt. Express16(24), 19821–19829 (2008). [CrossRef] [PubMed]
  10. P. Gao, B. Yao, I. Harder, N. Lindlein, and F. J. Torcal-Milla, “Phase-shifting Zernike phase contrast microscopy for quantitative phase measurement,” Opt. Lett.36(21), 4305–4307 (2011). [CrossRef] [PubMed]
  11. H. Kadono, M. Ogusu, and S. Toyooka, “Phase shifting common path interferometer using a liquid-crystal phase modulator,” Opt. Commun.110(3-4), 391–400 (1994). [CrossRef]
  12. G. Popescu, L. P. Deflores, J. C. Vaughan, K. Badizadegan, H. Iwai, R. R. Dasari, and M. S. Feld, “Fourier phase microscopy for investigation of biological structures and dynamics,” Opt. Lett.29(21), 2503–2505 (2004). [CrossRef] [PubMed]
  13. N. Lue, W. Choi, G. Popescu, T. Ikeda, R. R. Dasari, K. Badizadegan, and M. S. Feld, “Quantitative phase imaging of live cells using fast Fourier phase microscopy,” Appl. Opt.46(10), 1836–1842 (2007). [CrossRef] [PubMed]
  14. J. Glückstad and P. C. Mogensen, “Optimal Phase Contrast in Common-Path Interferometry,” Appl. Opt.40(2), 268–282 (2001). [CrossRef] [PubMed]
  15. S. Wolfling, E. Lanzmann, M. Israeli, N. Ben-Yosef, and Y. Arieli, “Spatial phase-shift interferometry—a wavefront analysis technique for three-dimensional topometry,” J. Opt. Soc. Am. A22(11), 2498–2509 (2005). [CrossRef]
  16. P. T. Samsheerali, B. Das, and J. Joseph, “Quantitative phase contrast imaging using common-path in-line digital holography,” Opt. Commun.285, 1062–1065 (2012). [CrossRef]
  17. D. Palima and J. Glückstad, “Diffractive generalized phase contrast for adaptive phase imaging and optical security,” Opt. Express20(2), 1370–1377 (2012). [CrossRef] [PubMed]
  18. P. J. Rodrigo, D. Palima, and J. Glückstad, “Accurate quantitative phase imaging using generalized phase contrast,” Opt. Express16(4), 2740–2751 (2008). [CrossRef] [PubMed]

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