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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 7, Iss. 9 — Aug. 28, 2012

Light sheet-excited spontaneous Raman imaging of a living fish by optical sectioning in a wide field Raman microscope

Yusuke Oshima, Hidetoshi Sato, Hiroko Kajiura-Kobayashi, Tetsuaki Kimura, Kiyoshi Naruse, and Shigenori Nonaka  »View Author Affiliations


Optics Express, Vol. 20, Issue 15, pp. 16195-16204 (2012)
http://dx.doi.org/10.1364/OE.20.016195


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Abstract

Spontaneous Raman microscopy is a potentially useful technique for imaging living cells, tissue and small animals without any probe or dye labeling. We have developed a spontaneous Raman imaging system in wide-field view, which we term ‘light sheet-excited direct Raman spectroscopy’ (LSDRS). This system, which we reported previously, consists of a background-free electrically tunable Ti:Sapphire laser (BF-ETL), a cylindrical lens, a CCD camera, and a narrow bandpass filter. Here, we have adapted the LSDRS system for microscopy systems, such as single-plane illumination microscopy (SPIM) for biomedical applications, and demonstrated spontaneous Raman imaging of a living fish. The results suggest that our Raman microscopy system enables investigation of the differentiation process and mechanism of iridocytes during development. This is the first report in which Raman imaging of a living animal was successfully demonstrated by spontaneous Raman scattering signals, but not nonlinear Raman effects such as CARS and SRS.

© 2012 OSA

OCIS Codes
(110.0110) Imaging systems : Imaging systems
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(300.6450) Spectroscopy : Spectroscopy, Raman
(110.4234) Imaging systems : Multispectral and hyperspectral imaging
(180.5655) Microscopy : Raman microscopy

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: April 11, 2012
Revised Manuscript: June 15, 2012
Manuscript Accepted: June 19, 2012
Published: July 2, 2012

Virtual Issues
Vol. 7, Iss. 9 Virtual Journal for Biomedical Optics

Citation
Yusuke Oshima, Hidetoshi Sato, Hiroko Kajiura-Kobayashi, Tetsuaki Kimura, Kiyoshi Naruse, and Shigenori Nonaka, "Light sheet-excited spontaneous Raman imaging of a living fish by optical sectioning in a wide field Raman microscope," Opt. Express 20, 16195-16204 (2012)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=oe-20-15-16195


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