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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 7, Iss. 9 — Aug. 28, 2012

Optimal laser scan path for localizing a fluorescent particle in two or three dimensions

Gregg M. Gallatin and Andrew J. Berglund  »View Author Affiliations

Optics Express, Vol. 20, Issue 15, pp. 16381-16393 (2012)

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Localizing a fluorescent particle by scanning a focused laser beam in its vicinity and analyzing the detected photon stream provides real-time information for a modern class of feedback control systems for particle tracking and trapping. We show for the full range of standard merit functions based on the Fisher information matrix (1) that the optimal path coincides with the positions of maximum slope of the square root of the beam intensity rather than with the intensity itself, (2) that this condition matches that derived from the theory describing the optimal design of experiments and (3) that in one dimension it is equivalent to maximizing the signal to noise ratio. The optimal path for a Gaussian beam scanned in two or three dimensions is presented along with the Cramér-Rao bound, which gives the ultimate localization accuracy that can be achieved by analyzing the detected photon stream. In two dimensions the optimum path is independent of the chosen merit function but this is not the case in three dimensions. Also, we show that whereas the optimum path for a Gaussian beam in two dimensions can be chosen to be continuous, it cannot be continuous in three dimensions.

© 2012 OSA

OCIS Codes
(180.2520) Microscopy : Fluorescence microscopy
(110.3055) Imaging systems : Information theoretical analysis

ToC Category:

Original Manuscript: May 17, 2012
Revised Manuscript: June 7, 2012
Manuscript Accepted: June 8, 2012
Published: July 3, 2012

Virtual Issues
Vol. 7, Iss. 9 Virtual Journal for Biomedical Optics

Gregg M. Gallatin and Andrew J. Berglund, "Optimal laser scan path for localizing a fluorescent particle in two or three dimensions," Opt. Express 20, 16381-16393 (2012)

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