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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 7, Iss. 9 — Aug. 28, 2012

Phasor analysis for nonlinear pump-probe microscopy

Francisco E. Robles, Jesse W. Wilson, Martin C. Fischer, and Warren S. Warren  »View Author Affiliations

Optics Express, Vol. 20, Issue 15, pp. 17082-17092 (2012)

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Pump-probe microscopy provides molecular information by probing transient, excited state dynamic properties of pigmented samples. Analysis of the transient response is typically conducted using principal component analysis or multi-exponential fitting, however these methods are not always practical or feasible. Here, we show an adaptation of phasor analysis to provide an intuitive, robust, and efficient method for analyzing and displaying pump-probe images, thereby alleviating some of the challenges associated with differentiating multiple pigments. A theoretical treatment is given to understand how the complex transient signals map onto the phasor plot. Analyses of cutaneous and ocular pigmented tissue samples, as well as historical pigments in art demonstrate the utility of this approach.

© 2012 OSA

OCIS Codes
(100.0100) Image processing : Image processing
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(190.7110) Nonlinear optics : Ultrafast nonlinear optics
(300.6420) Spectroscopy : Spectroscopy, nonlinear
(180.4315) Microscopy : Nonlinear microscopy

ToC Category:

Original Manuscript: June 7, 2012
Revised Manuscript: July 7, 2012
Manuscript Accepted: July 7, 2012
Published: July 11, 2012

Virtual Issues
Vol. 7, Iss. 9 Virtual Journal for Biomedical Optics

Francisco E. Robles, Jesse W. Wilson, Martin C. Fischer, and Warren S. Warren, "Phasor analysis for nonlinear pump-probe microscopy," Opt. Express 20, 17082-17092 (2012)

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