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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 7, Iss. 9 — Aug. 28, 2012

Simultaneous X-ray fluorescence and ptychographic microscopy of Cyclotella meneghiniana

D. J. Vine, D. Pelliccia, C. Holzner, S. B. Baines, A. Berry, I. McNulty, S. Vogt, A. G. Peele, and K. A. Nugent  »View Author Affiliations


Optics Express, Vol. 20, Issue 16, pp. 18287-18296 (2012)
http://dx.doi.org/10.1364/OE.20.018287


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Abstract

Scanning X-ray fluorescence microscopy (XFM) is a particularly useful method for studying the spatial distribution of trace metals in biological samples. Here we demonstrate the utility of combining coherent diffractive imaging (CDI) with XFM for imaging biological samples to simultaneously produce high-resolution and high-contrast transmission images and quantitative elemental maps. The reconstructed transmission function yields morphological details which contextualise the elemental maps. We report enhancement of the spatial resolution in both the transmission and fluorescence images beyond that of the X-ray optics. The freshwater diatom Cyclotella meneghiniana was imaged to demonstrate the benefits of combining these techniques that have complementary contrast mechanisms.

© 2012 OSA

OCIS Codes
(180.5810) Microscopy : Scanning microscopy
(180.7460) Microscopy : X-ray microscopy
(300.6560) Spectroscopy : Spectroscopy, x-ray
(340.7440) X-ray optics : X-ray imaging
(340.7460) X-ray optics : X-ray microscopy

ToC Category:
Microscopy

History
Original Manuscript: May 14, 2012
Revised Manuscript: June 21, 2012
Manuscript Accepted: June 22, 2012
Published: July 25, 2012

Virtual Issues
Vol. 7, Iss. 9 Virtual Journal for Biomedical Optics

Citation
D. J. Vine, D. Pelliccia, C. Holzner, S. B. Baines, A. Berry, I. McNulty, S. Vogt, A. G. Peele, and K. A. Nugent, "Simultaneous X-ray fluorescence and ptychographic microscopy of Cyclotella meneghiniana," Opt. Express 20, 18287-18296 (2012)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=oe-20-16-18287


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