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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 7, Iss. 9 — Aug. 28, 2012

Real-time monitoring of the solution concentration variation during the crystallization process of protein-lysozyme by using digital holographic interferometry

Yanyan Zhang, Jianlin Zhao, Jianglei Di, Hongzhen Jiang, Qian Wang, Jun Wang, Yunzhu Guo, and Dachuan Yin  »View Author Affiliations

Optics Express, Vol. 20, Issue 16, pp. 18415-18421 (2012)

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We report a real-time measurement method of the solution concentration variation during the growth of protein-lysozyme crystals based on digital holographic interferometry. A series of holograms containing the information of the solution concentration variation in the whole crystallization process is recorded by CCD. Based on the principle of double-exposure holographic interferometry and the relationship between the phase difference of the reconstructed object wave and the solution concentration, the solution concentration variation with time for arbitrary point in the solution can be obtained, and then the two-dimensional concentration distribution of the solution during crystallization process can also be figured out under the precondition which the refractive index is constant through the light propagation direction. The experimental results turns out that it is feasible to in situ, full-field and real-time monitor the crystal growth process by using this method.

© 2012 OSA

OCIS Codes
(120.5050) Instrumentation, measurement, and metrology : Phase measurement
(260.1180) Physical optics : Crystal optics
(090.1995) Holography : Digital holography
(090.5694) Holography : Real-time holography

ToC Category:
Instrumentation, Measurement, and Metrology

Original Manuscript: June 7, 2012
Revised Manuscript: July 15, 2012
Manuscript Accepted: July 16, 2012
Published: July 26, 2012

Virtual Issues
Vol. 7, Iss. 9 Virtual Journal for Biomedical Optics

Yanyan Zhang, Jianlin Zhao, Jianglei Di, Hongzhen Jiang, Qian Wang, Jun Wang, Yunzhu Guo, and Dachuan Yin, "Real-time monitoring of the solution concentration variation during the crystallization process of protein-lysozyme by using digital holographic interferometry," Opt. Express 20, 18415-18421 (2012)

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