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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 7, Iss. 10 — Oct. 5, 2012

Reconstruction of objects above and below the objective focal plane with dimensional fidelity by FINCH fluorescence microscopy

Nisan Siegel, Joseph Rosen, and Gary Brooker  »View Author Affiliations

Optics Express, Vol. 20, Issue 18, pp. 19822-19835 (2012)

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Fresnel Incoherent Correlation Holography (FINCH) can faithfully reproduce objects above and below the optical plane of focus. However, as in optical imaging, the transverse magnification and optimal reconstruction depth changes based on the longitudinal distance of objects from the focal plane of the input lens with the exception that objects above and below the focal plane are in focus with FINCH and out of focus by standard optical imaging. We have analyzed these effects both theoretically and experimentally for two configurations of a FINCH fluorescence microscopy system. This information has been used to reconstruct a test planar object placed above or below the optical plane of focus with high dimensional and image fidelity. Because FINCH is inherently a super-resolving system, this advance makes it possible to create super-resolved 3D images from FINCH holograms.

© 2012 OSA

OCIS Codes
(090.1760) Holography : Computer holography
(090.1970) Holography : Diffractive optics
(090.2880) Holography : Holographic interferometry
(100.6890) Image processing : Three-dimensional image processing
(110.0180) Imaging systems : Microscopy
(110.6880) Imaging systems : Three-dimensional image acquisition
(180.2520) Microscopy : Fluorescence microscopy
(180.6900) Microscopy : Three-dimensional microscopy
(260.2510) Physical optics : Fluorescence
(090.1995) Holography : Digital holography

ToC Category:

Original Manuscript: July 9, 2012
Revised Manuscript: August 2, 2012
Manuscript Accepted: August 3, 2012
Published: August 14, 2012

Virtual Issues
Vol. 7, Iss. 10 Virtual Journal for Biomedical Optics

Nisan Siegel, Joseph Rosen, and Gary Brooker, "Reconstruction of objects above and below the objective focal plane with dimensional fidelity by FINCH fluorescence microscopy," Opt. Express 20, 19822-19835 (2012)

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  1. J. Rosen and G. Brooker, “Digital spatially incoherent Fresnel holography,” Opt. Lett.32(8), 912–914 (2007). [CrossRef] [PubMed]
  2. B. Katz and J. Rosen, “Could SAFE concept be applied for designing a new synthetic aperture telescope?” Opt. Express19(6), 4924–4936 (2011). [CrossRef] [PubMed]
  3. M. K. Kim, “Adaptive optics by incoherent digital holography,” Opt. Lett.37(13), 2694–2696 (2012). [CrossRef] [PubMed]
  4. J. Rosen and G. Brooker, “Non-scanning motionless fluorescence three-dimensional holographic microscopy,” Nat. Photonics2(3), 190–195 (2008). [CrossRef]
  5. G. Brooker, N. Siegel, V. Wang, and J. Rosen, “Optimal resolution in Fresnel incoherent correlation holographic fluorescence microscopy,” Opt. Express19(6), 5047–5062 (2011). [CrossRef] [PubMed]
  6. J. Rosen, N. Siegel, and G. Brooker, “Theoretical and experimental demonstration of resolution beyond the Rayleigh limit by FINCH fluorescence microscopic imaging,” Opt. Express19(27), 26249–26268 (2011). [CrossRef] [PubMed]
  7. P. Bouchal, J. Kapitán, R. Chmelík, and Z. Bouchal, “Point spread function and two-point resolution in Fresnel incoherent correlation holography,” Opt. Express19(16), 15603–15620 (2011). [CrossRef] [PubMed]
  8. X. Lai, Y. Zhao, X. Lv, Z. Zhou, and S. Zeng, “Fluorescence holography with improved signal-to-noise ratio by near image plane recording,” Opt. Lett.37(13), 2445–2447 (2012). [CrossRef] [PubMed]
  9. B. Katz, J. Rosen, R. Kelner, and G. Brooker, “Enhanced resolution and throughput of Fresnel incoherent correlation holography (FINCH) using dual diffractive lenses on a spatial light modulator (SLM),” Opt. Express20(8), 9109–9121 (2012). [CrossRef] [PubMed]
  10. I. Yamaguchi and T. Zhang, “Phase-shifting digital holography,” Opt. Lett.22(16), 1268–1270 (1997). [CrossRef] [PubMed]

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