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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 7, Iss. 10 — Oct. 5, 2012

Angiography of the retina and the choroid with phase-resolved OCT using interval-optimized backstitched B-scans

Boy Braaf, Koenraad A. Vermeer, Kari V. Vienola, and Johannes F. de Boer  »View Author Affiliations


Optics Express, Vol. 20, Issue 18, pp. 20516-20534 (2012)
http://dx.doi.org/10.1364/OE.20.020516


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Abstract

In conventional phase-resolved OCT blood flow is detected from phase changes between successive A-scans. Especially in high-speed OCT systems this results in a short evaluation time interval. This method is therefore often unable to visualize complete vascular networks since low flow velocities cause insufficient phase changes. This problem was solved by comparing B-scans instead of successive A-scans to enlarge the time interval. In this paper a detailed phase-noise analysis of our OCT system is presented in order to calculate the optimal time intervals for visualization of the vasculature of the human retina and choroid. High-resolution images of the vasculature of a healthy volunteer taken with various time intervals are presented to confirm this analysis. The imaging was performed with a backstitched B-scan in which pairs of small repeated B-scans are stitched together to independently control the time interval and the imaged lateral field size. A time interval of ≥2.5 ms was found effective to image the retinal vasculature down to the capillary level. The higher flow velocities of the choroid allowed a time interval of 0.64 ms to reveal its dense vasculature. Finally we analyzed depth-resolved histograms of volumetric phase-difference data to assess changes in amount of blood flow with depth. This analysis indicated different flow regimes in the retina and the choroid.

© 2012 OSA

OCIS Codes
(110.0110) Imaging systems : Imaging systems
(110.4500) Imaging systems : Optical coherence tomography
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.4470) Medical optics and biotechnology : Ophthalmology
(280.2490) Remote sensing and sensors : Flow diagnostics

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: June 6, 2012
Revised Manuscript: August 13, 2012
Manuscript Accepted: August 13, 2012
Published: August 22, 2012

Virtual Issues
Vol. 7, Iss. 10 Virtual Journal for Biomedical Optics

Citation
Boy Braaf, Koenraad A. Vermeer, Kari V. Vienola, and Johannes F. de Boer, "Angiography of the retina and the choroid with phase-resolved OCT using interval-optimized backstitched B-scans," Opt. Express 20, 20516-20534 (2012)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=oe-20-18-20516


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