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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 7, Iss. 11 — Oct. 31, 2012

Phase shifting technique for extended inline holographic microscopy with a pinhole array

Christian Graulig, Mario Kanka, and Rainer Riesenberg  »View Author Affiliations


Optics Express, Vol. 20, Issue 20, pp. 22383-22390 (2012)
http://dx.doi.org/10.1364/OE.20.022383


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Abstract

Digital inline holographic microscopy using a pinhole for sample illumination allows lensless imaging. To overcome restrictions of the sample size and density in the setup additional reference waves are generated by extending the single pinhole to a regular 2D pinhole array illumination. A technique is presented that uses phase shifting between the pinhole waves. Multiple foci with stable phase differences and a phase error (rms) of 0.027 rad generate pinhole waves which illuminate an undiluted, dense blood smear sample. Amplitude and phase images of the blood sample were sucessfully reconstructed.

© 2012 OSA

OCIS Codes
(180.0180) Microscopy : Microscopy
(090.1995) Holography : Digital holography
(070.6120) Fourier optics and signal processing : Spatial light modulators

ToC Category:
Microscopy

History
Original Manuscript: July 12, 2012
Revised Manuscript: August 29, 2012
Manuscript Accepted: September 9, 2012
Published: September 17, 2012

Virtual Issues
Vol. 7, Iss. 11 Virtual Journal for Biomedical Optics

Citation
Christian Graulig, Mario Kanka, and Rainer Riesenberg, "Phase shifting technique for extended inline holographic microscopy with a pinhole array," Opt. Express 20, 22383-22390 (2012)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=oe-20-20-22383


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