OSA's Digital Library

Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 7, Iss. 11 — Oct. 31, 2012

Digital confocal microscope

Alexandre S. Goy and Demetri Psaltis  »View Author Affiliations


Optics Express, Vol. 20, Issue 20, pp. 22720-22727 (2012)
http://dx.doi.org/10.1364/OE.20.022720


View Full Text Article

Enhanced HTML    Acrobat PDF (2501 KB) Open Access





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

We demonstrate experimentally a scanning confocal microscopy technique based on digital holography. The method relies on digital holographic recording of the scanned spot. The data collected in this way contains all the necessary information to digitally produce three-dimensional images. Several methods to treat the data are presented. Examples of reflection and transmission images of epithelial cells and mouse brain tissue are shown.

© 2012 OSA

OCIS Codes
(180.1790) Microscopy : Confocal microscopy
(090.1995) Holography : Digital holography

ToC Category:
Microscopy

History
Original Manuscript: July 6, 2012
Revised Manuscript: September 7, 2012
Manuscript Accepted: September 15, 2012
Published: September 19, 2012

Virtual Issues
Vol. 7, Iss. 11 Virtual Journal for Biomedical Optics

Citation
Alexandre S. Goy and Demetri Psaltis, "Digital confocal microscope," Opt. Express 20, 22720-22727 (2012)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=oe-20-20-22720


Sort:  Author  |  Year  |  Journal  |  Reset  

References

  1. M. Minsky, “Microscopy apparatus,” US Patent 3,013,467 (1961).
  2. C. Sheppard and D. Shotton, Confocal Laser Scanning Microscopy (Oxford, BIOS Scientific Publishers 1997).
  3. Y. C. Liu and A. S. Chiang, “High-resolution confocal imaging and three-dimensional rendering,” Methods30(1), 86–93 (2003). [CrossRef] [PubMed]
  4. S. J. Tseng, Y. H. Lee, Z. H. Chen, H. H. Lin, C. Y. Lin, and S. C. Tang, “Integration of optical clearing and optical sectioning microscopy for three-dimensional imaging of natural biomaterial scaffolds in thin sections,” J. Biomed. Opt.14(4), 044004 (2009). [CrossRef] [PubMed]
  5. M. J. Booth, “Adaptive optics in microscopy,” Philos. Transact. A Math. Phys. Eng. Sci.365(1861), 2829–2843 (2007). [CrossRef] [PubMed]
  6. J. M. Girkin, S. Poland, and A. J. Wright, “Adaptive optics for deeper imaging of biological samples,” Curr. Opin. Biotechnol.20(1), 106–110 (2009). [CrossRef] [PubMed]
  7. F. Charrière, A. Marian, F. Montfort, J. Kuehn, T. Colomb, E. Cuche, P. Marquet, and C. Depeursinge, “Cell refractive index tomography by digital holographic microscopy,” Opt. Lett.31(2), 178–180 (2006). [CrossRef] [PubMed]
  8. K. Dillon and Y. Fainman, “Computational confocal tomography for simultaneous reconstruction of objects, occlusions, and aberrations,” Appl. Opt.49(13), 2529–2538 (2010). [CrossRef]
  9. N. Lue, W. Choi, K. Badizadegan, R. R. Dasari, M. S. Feld, and G. Popescu, “Confocal diffraction phase microscopy of live cells,” Opt. Lett.33(18), 2074–2076 (2008). [CrossRef] [PubMed]
  10. G. Barbastathis, M. Balberg, and D. J. Brady, “Confocal microscopy with a volume holographic filter,” Opt. Lett.24(12), 811–813 (1999). [CrossRef] [PubMed]
  11. C. Yang and J. Mertz, “Transmission confocal laser scanning microscopy with a virtual pinhole based on nonlinear detection,” Opt. Lett.28(4), 224–226 (2003). [CrossRef] [PubMed]
  12. E. Cuche, P. Marquet, and C. Depeursinge, “Spatial filtering for zero-order and twin-image elimination in digital off-axis holography,” Appl. Opt.39(23), 4070–4075 (2000). [CrossRef] [PubMed]
  13. S. G. Johnson and M. Frigo, “A modified split-radix FFT with fewer arithmetic operations,” IEEE Trans. Signal Process.55(1), 111–119 (2007). [CrossRef]
  14. D. J. Brady, K. Choi, D. L. Marks, R. Horisaki, and S. Lim, “Compressive holography,” Opt. Express17(15), 13040–13049 (2009). [CrossRef] [PubMed]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.


« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited