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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 8, Iss. 1 — Feb. 4, 2013

Fluorescence lifetime imaging with pulsed diode laser enabled stimulated emission

Jianhong Ge, Cuifang Kuang, Shin-Shian Lee, and Fu-Jen Kao  »View Author Affiliations


Optics Express, Vol. 20, Issue 27, pp. 28216-28221 (2012)
http://dx.doi.org/10.1364/OE.20.028216


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Abstract

We present here a stimulated emission based fluorescence lifetime imaging (FLIM) scheme using a pair of synchronized diode lasers operating at gain switched pulse mode. The two semiconductor lasers, with wavelengths at 635 nm and 700 nm, serve as the excitation and the stimulation light sources for the ATTO647N labeled sample, respectively. FLIM is readily achieved with their relative time delay controlled electronically. The coherent nature of the stimulated emission signal also allows FLIM at long working distance. In this way, a high performance all-semiconductor FLIM module is realized in a flexible, compact, and cost effective configuration.

© 2012 OSA

OCIS Codes
(170.1650) Medical optics and biotechnology : Coherence imaging
(170.6920) Medical optics and biotechnology : Time-resolved imaging
(260.2510) Physical optics : Fluorescence

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: September 20, 2012
Revised Manuscript: November 11, 2012
Manuscript Accepted: November 20, 2012
Published: December 5, 2012

Virtual Issues
Vol. 8, Iss. 1 Virtual Journal for Biomedical Optics

Citation
Jianhong Ge, Cuifang Kuang, Shin-Shian Lee, and Fu-Jen Kao, "Fluorescence lifetime imaging with pulsed diode laser enabled stimulated emission," Opt. Express 20, 28216-28221 (2012)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=oe-20-27-28216


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