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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 8, Iss. 1 — Feb. 4, 2013

On the holographic 3D tracking of in vitro cells characterized by a highly-morphological change

Pasquale Memmolo, Maria Iannone, Maurizio Ventre, Paolo Antonio Netti, Andrea Finizio, Melania Paturzo, and Pietro Ferraro  »View Author Affiliations


Optics Express, Vol. 20, Issue 27, pp. 28485-28493 (2012)
http://dx.doi.org/10.1364/OE.20.028485


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Abstract

Digital Holography (DH) in microscopic configuration is a powerful tool for the imaging of micro-objects contained into a three dimensional (3D) volume, by a single-shot image acquisition. Many studies report on the ability of DH to track particle, microorganism and cells in 3D. However, very few investigations are performed with objects that change severely their morphology during the observation period. Here we study DH as a tool for 3D tracking an osteosarcoma cell line for which extensive changes in cell morphology are associated to cell motion. Due to the great unpredictable morphological change, retrieving cell’s position in 3D can become a complicated issue. We investigate and discuss in this paper how the tridimensional position can be affected by the continuous change of the cells. Moreover we propose and test some strategies to afford the problems and compare it with others approaches. Finally, results on the 3D tracking and comments are reported and illustrated.

© 2012 OSA

OCIS Codes
(180.3170) Microscopy : Interference microscopy
(180.6900) Microscopy : Three-dimensional microscopy
(090.1995) Holography : Digital holography

ToC Category:
Microscopy

History
Original Manuscript: October 10, 2012
Revised Manuscript: October 22, 2012
Manuscript Accepted: October 22, 2012
Published: December 7, 2012

Virtual Issues
Vol. 8, Iss. 1 Virtual Journal for Biomedical Optics

Citation
Pasquale Memmolo, Maria Iannone, Maurizio Ventre, Paolo Antonio Netti, Andrea Finizio, Melania Paturzo, and Pietro Ferraro, "On the holographic 3D tracking of in vitro cells characterized by a highly-morphological change," Opt. Express 20, 28485-28493 (2012)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=oe-20-27-28485


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