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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 8, Iss. 1 — Feb. 4, 2013

Measurement of object structure from size-encoded images generated by optically-implemented Gabor filters

Heidy Sierra, Jing-Yi Zheng, Bryan Rabin, and Nada N. Boustany  »View Author Affiliations


Optics Express, Vol. 20, Issue 27, pp. 28698-28706 (2012)
http://dx.doi.org/10.1364/OE.20.028698


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Abstract

We use optical Fourier processing based on two dimensional (2D) Gabor filters to obtain size-encoded images which depict with 20nm sensitivity to size while preserving a 0.36μm spatial resolution, the spatial distribution of structural features within transparent objects. The size of the object feature measured at each pixel in the encoded image is determined by the optimal Gabor filter period, Smax, that maximizes the scattering signal from that location in the object. We show that Smax (in μm) depends linearly on feature size (also in μm) over a size range from 0.11μm to 2μm. This linear response remains largely unchanged when the refractive index ratio is varied and can be predicted from numerical simulations of Gabor-filtered light scattering. Pixel histograms of the size-encoded images of isolated spheres and diatoms were used to generate highly resolved size distributions (“size spectra”) exhibiting sharp peaks characterizing the known major structural features within the studied objects. Dynamic signal associated with changes in selected feature sizes within living cells is also demonstrated. Taken together, our data suggest that a label-free, direct and objective measurement of sample structure is enabled by the size-encoded images and associated pixel histograms generated from a calibrated optical processing microscope based on Gabor filtering.

© 2012 OSA

OCIS Codes
(070.4790) Fourier optics and signal processing : Spectrum analysis
(170.1420) Medical optics and biotechnology : Biology
(170.1530) Medical optics and biotechnology : Cell analysis
(290.5820) Scattering : Scattering measurements
(070.2615) Fourier optics and signal processing : Frequency filtering

ToC Category:
Image Processing

History
Original Manuscript: October 9, 2012
Manuscript Accepted: November 12, 2012
Published: December 10, 2012

Virtual Issues
Vol. 8, Iss. 1 Virtual Journal for Biomedical Optics

Citation
Heidy Sierra, Jing-Yi Zheng, Bryan Rabin, and Nada N. Boustany, "Measurement of object structure from size-encoded images generated by optically-implemented Gabor filters," Opt. Express 20, 28698-28706 (2012)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=oe-20-27-28698


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